Evidence map›Paper›PMID 40505661›Full record

ArticleCell chemical biology2025

A cationic amphiphilic drug synergizes with strobilurin fungicides to control fungal-borne plant diseases.

Bradley Laflamme, Christopher Blackman, Mackenzie Loranger, Richard Trilles, Kalina Doytchinova-Weil, Stephen S Scully, J Miles Blackburn, Anastasia L G Kanegesuku, Jennifer L Roizen, Stephen Bengtson and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Bradley LaflammeDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Christopher BlackmanAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada.
Mackenzie LorangerDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada.
Richard TrillesDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
Kalina Doytchinova-WeilDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
Stephen S ScullyDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
J Miles BlackburnDepartment of Chemistry, Duke University, Durham, NC 27708, USA.
Anastasia L G KanegesukuDepartment of Chemistry, Duke University, Durham, NC 27708, USA.
Jennifer L RoizenDepartment of Chemistry, Duke University, Durham, NC 27708, USA.
Stephen BengtsonDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
Lauren E BrownDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
John A PorcoDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
Keiko YoshiokaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada.
Luke WhitesellDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Rajagopal SubramaniamAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.
Nicole RobbinsDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Leah E CowenDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address: leah.cowen@utoronto.ca.

Funding

Chemical Synthesis of Complex Natural Products for Translational ScienceR35GM118173 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI JOHN A. PORCO · 2016 to 2026
$8.2M
BU-CMD Chemical Library Consortium: Fostering Collaborations between Chemists and Biologists for Translational DiscoveryU01TR002625 · NCATS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI PORCO, JOHN A. · 2020 to 2023
$2.7M
Exploiting Masked Alcohols to Guide Atom-Transfer ReactionsR35GM128741 · NIGMS · DUKE UNIVERSITY · PI ROIZEN, JENNIFER L · 2018 to 2020
$1.2M
NCATS NIH HHS U01 TR002625NIGMS NIH HHS R35 GM118173NIGMS NIH HHS R35 GM128741
6 · The paper itself

Abstract

Fungal phytopathogens are responsible for major losses in agricultural yields annually. While the use of topical fungicides remains key to managing agricultural pathogens, the emergence of drug-resistant strains necessitates identifying additional treatment options. In this study, we performed an in vitro small molecule screen against the devastating cereal pathogen Fusarium graminearum, identifying CMLD009688 as a priority growth inhibitor. Chemical-genetic profiling and subsequent experiments with CMLD009688 revealed that this compound functions as a cationic amphiphilic drug (CAD) and perturbs vacuolar integrity in F. graminearum. CMLD009688 displayed synergy with strobilurin fungicides in limiting F. graminearum growth-likely through both compounds independently affecting vacuolar stability-and a combination treatment of CMLD009688 with the strobilurin pyraclostrobin strongly limited the virulence of both F. graminearum and Botrytis cinerea in distinct models of plant infection. Thus, our findings highlight that cationic amphiphilic molecules show immense promise in helping to protect crops from fungal diseases.

Indexed as

Fungicides, IndustrialFusariumPlant DiseasesStrobilurinsSurface-Active AgentsBotrytisCationsDrug SynergismMicrobial Sensitivity TestsCationsFungicides, IndustrialStrobilurinsSurface-Active AgentsBotrytis cinearacationic amphiphilic drugschemical biologychemogenomicsfungal pathogenesisfungicideFusarium graminearumpyraclostrobinstrobilurin

Identifiers

PMID40505661
PMCPMC12212970

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.