Evidence map›Paper›PMID 40504295›Full record

ArticleMedical oncology (Northwood, London, England)2025

KLF4 mediates the contradictory effects of phenylethyl isothiocyanate in gastric cancer intervention.

Qi Zhang, Chenying Yang, Bei Zhou, Xingsu Gao, Caiyun Zhong, Ye Shi, Hui Cao, Mingming Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Medical oncology (Northwood, London, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qi Zhang *Department of Public Health, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Chenying Yang *Yinzhou Center for Disease Control and Prevention, Ningbo, 315000, China.
Bei ZhouDepartment of Nutrition, School of Acupuncture and Tuina, Nanjing University of Chinese Medicine, 138, Xianling Avenue, Qixia District, Nanjing, 210023, China.
Xingsu GaoDepartment of Public Health, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Caiyun ZhongDepartment of Nutrition and Food Safety, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China.
Ye ShiDepartment of Thoracic Surgery, Nanjing Chest Hospital, Affiliated Nanjing Brain Hospital, Nanjing Medical University, 264, Guangzhou Road, Nanjing, 210029, China. docshiye@njmu.edu.cn.
Hui CaoDepartment of Thoracic Surgery, Nanjing Chest Hospital, Affiliated Nanjing Brain Hospital, Nanjing Medical University, 264, Guangzhou Road, Nanjing, 210029, China. doccaohui@njmu.edu.cn.
Mingming ZhuDepartment of Nutrition, School of Acupuncture and Tuina, Nanjing University of Chinese Medicine, 138, Xianling Avenue, Qixia District, Nanjing, 210023, China. mmzhu@njucm.edu.cn.

Funding

Health Science Development Foundation of Nanjing, China YKK23137Jiangsu Province "The 14th Five-year Plan" Key Discipline-Public Health and Preventive Medicine 035091005007National Natural Science Foundation of China 81803217Youth Talent Training Program of the Affiliated Nanjing Brain Hospital, Nanjing Medical University 23-25-2R21
6 · The paper itself

Abstract

Phenethyl isothiocyanate (PEITC) exerts anti-gastric cancer effect, however, the molecular mechanism has not yet been elucidated. KLF4 is an important regulatory molecule in gastric cancer. The programmed death ligand 1 (PD-L1) interacts with programmed cell death 1 (PD-1), inhibits function of cytotoxic T-lymphocyte and helps gastric cancer cells evade immune surveillance. The present study aimed to investigate the effect of PEITC against gastric cancer and the roles of KLF4 and PD-L1 in the anti-gastric cancer effect of PEITC. The effects of PEITC on gastric cancer cell proliferation and apoptosis were detected by EdU assay, flow cytometric analysis, immunoblotting and in vivo xenograft tumor experiment. The expression of KLF4 and PD-L1 was examined in PEITC-treated gastric cancer cells using immunoblotting, immunofluorescence and flow cytometric analysis. Co-culture system was used to assess the cancer cell-killing effect of PEITC combined with anti-PD-L1 blockade in gastric cancer. The results showed that PEITC suppressed cell activities both in vitro and in vivo. PEITC upregulated the expression of KLF4, which suppressed cyclin D1 expression and activated Bax expression, and mediated the growth inhibition effect of PEITC in gastric cancer. Meanwhile, we found that KLF4 induced by PEITC transcriptionally activated PD-L1 expression and diminished anti-cancer effects of anti-PD-L1 therapy in gastric cancer cells. Findings from this research revealed that PEITC restricted gastric cancer cell growth through activating KLF4. PEITC attenuated anti-PD-L1 therapy efficacy attributing to KLF4-upregulated PD-L1. These results uncover a novel mechanism of PEITC in gastric cancer intervention.

Indexed as

IsothiocyanatesKruppel-Like Transcription FactorsStomach NeoplasmsAnimalsApoptosisB7-H1 AntigenCell Line, TumorCell ProliferationHumansKruppel-Like Factor 4MiceMice, Inbred BALB CMice, NudeXenograft Model Antitumor AssaysB7-H1 AntigenCD274 protein, humanIsothiocyanatesKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription Factorsphenethyl isothiocyanateGastric cancerKLF4PD-L1Phenethyl isothiocyanate

Identifiers

PMID40504295

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.