Evidence map›Paper›PMID 40504260›Full record

ArticleApplied microbiology and biotechnology2025

3C protease-independent production of foot-and-mouth disease virus-like particles in Pichia pastoris.

Zhiyao Li, Hu Dong, Shuanghui Yin, Manyuan Bai, Yun Zhang, Yaozhong Ding, Shiqi Sun, Huichen Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhiyao LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Hu DongState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shuanghui YinState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Manyuan BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yun ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yaozhong DingState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shiqi SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Huichen GuoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. guohuichen@caas.cn.ORCID http://orcid.org/0000-0003-0539-497X

Funding

Key R&D program of Ningxia province 2024BBF02017technology innovation guidance program of gansu province 24CXNA030
6 · The paper itself

Abstract

The inactivated vaccines have played a pivotal role in the control and eradication of foot-and-mouth disease (FMD). However, certain safety concerns remain. Recently, virus-like particles (VLPs) have gradually become a research hotspot. As the eukaryotic expression system with the lowest production costs, the production of VLPs using Pichia pastoris has significant potential. During the natural infection process of FMD virus (FMDV), the polyprotein P1 is cleaved by 3C protease to form VP0, VP3, and VP1, which are subsequently assembled into VLPs. In this study, we adopted an alternative approach, co-expressing VP0, VP3, and VP1 without 3C protease for the production of FMDV VLPs in P. pastoris. The western blot (WB) assays showed variable protein expression on the same plasmid. VP0 was the highest, while VP3 and VP1 were similar. Furthermore, the order of proteins on the plasmid also mattered. The results indicated that His

Indexed as

Cysteine EndopeptidasesFoot-and-Mouth Disease VirusPichiaSaccharomycetalesVaccines, Virus-Like ParticleViral Proteins3C Viral ProteasesCapsid ProteinsRecombinant Proteins3C Viral ProteasesCapsid ProteinsCysteine EndopeptidasesRecombinant ProteinsVaccines, Virus-Like ParticleViral Proteins3C proteaseFoot-and-mouth diseasePichia pastorisPurificationVirus-like-particles

Identifiers

PMID40504260
PMCPMC12162760

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.