ArticleRSC advances2025
Discrepancies in qPCR-based gene quantification and their dependencies on soil properties, inhibitor presence, and DNA extraction kit types.
Article in RSC advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Progress of Rapid Detection Technology for Aquatic Microorganisms: A Comprehensive Review.Microorganisms · 2026Review
- Molecular and Culture-Based Surveillance of Free-Living Amoebae in Human Related Sources in an Outermost Region.Pathogens (Basel, Switzerland) · 2026Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complexity and heterogeneity of soil samples necessitate the inclusion of extensive purification steps prior to genomic assays, such as quantitative PCR (qPCR). Although conventional DNA extraction kits have notably enhanced the convenience of the process, those designed for soil vary considerably in terms of reagents, time, and equipment. Therefore, purified gDNA quality varies depending on the DNA extraction kits used, which leads to discrepancies in gene quantification using qPCR. This issue can be amplified considerably when more complicated (or contaminated) soils are analyzed, even if extensive DNA extraction is employed. Here, we evaluated the influences of the DNA extraction method to the gene quantification using qPCR across soil types. Further Mg
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Registered trials
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