Evidence map›Paper›PMID 40502972›Full record

ArticleACS catalysis2025

Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay.

Ina Somvilla, Hannes Meinert, Clemens Cziegler, Tobias Gökler, Christoph F Berner, Hannes Wolfgramm, Yannick Branson, Anne C Conibear, Uwe Völker, Christoffel P S Badenhorst and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS catalysis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Genuine Directed Evolution In Test Tube (GENie).bioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ina SomvillaDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0009-0000-7790-0566
Hannes MeinertDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0000-0001-6545-5047
Clemens CzieglerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0009-0005-3721-1922
Tobias GöklerInstitute of Applied Synthetic Chemistry, TU Wien, Vienna 1060, Austria.
Christoph F BernerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.
Hannes WolfgrammDepartment of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald 17475, Germany.ORCID https://orcid.org/0000-0002-6028-0046
Yannick BransonDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0000-0002-0282-7090
Anne C ConibearInstitute of Applied Synthetic Chemistry, TU Wien, Vienna 1060, Austria.ORCID https://orcid.org/0000-0002-5482-6225
Uwe VölkerDepartment of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald 17475, Germany.ORCID https://orcid.org/0000-0002-5689-3448
Christoffel P S BadenhorstDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0000-0002-5874-4577
Thomas BayerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0000-0002-0656-3280
Uwe T BornscheuerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald 17487, Germany.ORCID https://orcid.org/0000-0003-0685-2696

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ultrahigh-throughput methods such as flow cytometry are ideal tools for the directed evolution of enzymes by enabling the screening of up to 10

Indexed as

amidasebiocatalysisflow cytometryhigh-throughput screeningloop engineering

Identifiers

PMID40502972
PMCPMC12150331

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.