ArticleResearch square2025
An RNAi-based platform for spatiotemporal control of histone gene expression during animal development.
Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Mutational analysis of histones provides an important means of studying the function of histone post-translational modifications (PTMs) in epigenetic gene regulation. However, several technical challenges have impeded direct tests of histone residue function in metazoans, including the massive abundance of histone gene products, multiple copies of histone genes in the genome, and the necessity of histones for cell viability. Results: Here, we describe a new experimental approach in Conclusions: We conclude that shRNA-mediated depletion of endogenous histone H3.2, coupled with histone replacement transgenes engineered to be insensitive to RNAi, is an effective experimental approach for studying the role of histone PTMs in animal development.
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