Evidence map›Paper›PMID 40502699›Full record

ArticleiScience2025

Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.

Tadamoto Isogai, Kevin M Dean, Philippe Roudot, Evgenia V Azarova, Kushal Bhatt, Meghan K Driscoll, Shaina P Royer, Nikhil Mittal, Bo-Jui Chang, Sangyoon J Han and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tadamoto IsogaiLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Kevin M DeanLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Philippe RoudotLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Evgenia V AzarovaLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Kushal BhattLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Meghan K DriscollLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Shaina P RoyerDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
Nikhil MittalDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
Bo-Jui ChangDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Sangyoon J HanDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
Reto FiolkaLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Gaudenz DanuserLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Funding

UT Southwestern Medical Center Simmons Comprehensive Cancer CenterP30CA142543 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Sandi Leigh Pruitt · 2010 to 2026
$53.7M
Functional causality in regulating cell morphogenesisR35GM136428 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI DANUSER, GAUDENZ · 2020 to 2024
$4.1M
Nascent Adhesion-Based Mechano-transmission for Extracellular Matrix Stiffness SensingR15GM135806 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI HAN, SANGYOON JOSHUA · 2019 to 2024
$964k
Multiscale microscope for 3D cancer imaging in model organisms and organoidsR33CA235254 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI FIOLKA, RETO PAUL · 2019 to 2021
$930k
Formation and function of lamellipodial morphology in 3D microenvironmentsK99GM123221 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI DRISCOLL, MEGHAN KATRIEN · 2018 to 2019
$180k
Symmetry breaking and polarization of cell in 3D environmentsF32GM117793 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI DEAN, KEVIN MICHAEL · 2016 to 2017
$121k
Bleb Functions in 3D MorphogenesisF32GM116370 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI DRISCOLL, MEGHAN KATRIEN · 2015 to 2016
$118k
NCI NIH HHS P30 CA142543NCI NIH HHS R33 CA235254NIGMS NIH HHS F32 GM116370NIGMS NIH HHS F32 GM117793NIGMS NIH HHS K99 GM123221NIGMS NIH HHS R15 GM135806NIGMS NIH HHS R35 GM136428
6 · The paper itself

Abstract

A critical step in cell morphogenesis is the extension of actin-dense pseudopods, controlled by actin-binding proteins (ABPs). While this process is well-understood on glass coverslips, it is less so in compliant three-dimensional environments. Here, we knocked out a series of ABPs in osteosarcoma cells and evaluated their effect on pseudopod extension on glass surfaces (2D) and in collagen gels (3D). Cells lacking the longest Arp3 gene variant, or with attenuated Arp2/3 activity, had the strongest reduction in pseudopod formation between 2D and 3D. This was largely due to reduced activity of the hybrid Arp2/3-vinculin complex, which was dispensable on glass. Our data suggests that concurrent formation of actin branches

Indexed as

Biological morphologyCell biologyMolecular biology

Identifiers

PMID40502699
PMCPMC12152658

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.