Evidence map›Paper›PMID 40502090›Full record

ArticlebioRxiv : the preprint server for biology2025

Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly(Methyl Methacrylate)-Based Workflow.

David Bächinger, Brock Peyton, Jacqueline Neubauer, Anbuselvan Dharmarajan, MengYu Zhu, Jennifer T O'Malley, Venus Kallupurackal, Steven Senese, Alison Brown, Sabina Wunderlin and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

David BächingerDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, Zurich, Switzerland.
Brock PeytonOtopathology Laboratory, Mass Eye and Ear, MA, USA.
Jacqueline NeubauerZurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.ORCID 0000-0002-9472-7803
Anbuselvan DharmarajanOtopathology Laboratory, Mass Eye and Ear, MA, USA.
MengYu ZhuOtopathology Laboratory, Mass Eye and Ear, MA, USA.
Jennifer T O'MalleyOtopathology Laboratory, Mass Eye and Ear, MA, USA.
Venus KallupurackalZurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.
Steven SeneseDepartment of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Alison BrownLaboratory for Molecular Medicine, Personalized Medicine, Mass General Brigham, Cambridge, MA, USA.
Sabina WunderlinDepartment of Pathobiology, Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Susanne KreutzerFunctional Genomics Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland.
Nora M WeissDepartment of Otorhinolaryngology, Head and Neck Surgery "Otto Körner", University Medical Center, Rostock, Germany.ORCID 0000-0002-0645-3591
Heiko RichterLLS Rowiak LaserLabSolutions GmbH, Hannover, Germany.
Adrian DalbertDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, Zurich, Switzerland.ORCID 0000-0001-7714-8407
Christof RöösliDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital Zurich, Zurich, Switzerland.
Anja KiparDepartment of Pathobiology, Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.ORCID 0000-0001-7289-3459
Zsuzsanna VargaDepartment of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, Switzerland.
Brigitte von RechenbergMusculoskeletal Research Unit, Vetsuisse Faculty, University of Zurich, Switzerland.ORCID 0000-0002-3836-6812
Sami S AmrLaboratory for Molecular Medicine, Personalized Medicine, Mass General Brigham, Cambridge, MA, USA.ORCID 0000-0003-0927-6057
Andreas H EckhardOtopathology Laboratory, Mass Eye and Ear, MA, USA.ORCID 0000-0002-4921-6043

Funding

Translational Imaging and Phenotyping CoreP30AR075042 · NIAMS · MASSACHUSETTS GENERAL HOSPITAL · PI Marie Demay · 2019 to 2026
$7.4M
Massachusetts Eye and Ear/ Harvard Initiative for the NIDCD National Human Ear Resource NetworkU24DC020849 · NIDCD · MASSACHUSETTS EYE AND EAR INFIRMARY · PI M. Charles Liberman, Alicia M Quesnel · 2022 to 2026
$3.2M
NIAMS NIH HHS P30 AR075042NIDCD NIH HHS U24 DC020849
6 · The paper itself

Abstract

Hearing and balance disorders are the most prevalent sensory impairments, affecting hundreds of millions worldwide, yet their underlying cellular and molecular pathologies remain poorly understood. This knowledge gap stems from the inaccessibility of the ear's sensory organs-embedded within the temporal bone (TB), the hardest bone in the body-which cannot be biopsied in living patients without causing irreversible damage. Conventional histopathology workflows rely on postmortem en bloc extraction of TBs, followed by lengthy decalcification, celloidin embedding, and manual serial sectioning of these large specimens-a process that takes one to two years, is labor- and cost-intensive, and lacks compatibility with most modern protein, DNA, and RNA assays. Here, we present a rapid, reversible polymethyl methacrylate (rPMMA) workflow that enables advanced molecular histopathology studies on formalin-fixed, calcified TBs. Our protocol uses low-temperature (-40 °C to +4 °C) resin embedding, precision near-serial sectioning (10-50 µm) via femtosecond laser microtomy or precision diamond wire sawing, and subsequent deacrylation to fully restore tissue accessibility for high-fidelity histomorphology, multiplexed immunofluorescence, whole-genome sequencing, and in situ mRNA detection (RNAscope

Indexed as

celloidinDNAimmunohistochemistryinner earlaser microtomyreversible poly methyl methacrylateRNAscopeTechnovit® 9100

Identifiers

PMID40502090
PMCPMC12157654

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.