Evidence map›Paper›PMID 40501894›Full record

ArticlebioRxiv : the preprint server for biology2025

Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch.

Rosa A Romero, Adrien Chauvier, Serena S Teh, Vincent A Reed, Sicheng Zhang, Courtney E Szyjka, Shi-Jie Chen, Eric J Strobel, Nils G Walter

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Rosa A RomeroSingle Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Adrien ChauvierSingle Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Serena S TehDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.
Vincent A ReedSingle Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Sicheng ZhangDepartment of Physics and Astronomy, University of Missouri, Columbia, Missouri 65211, USA.
Courtney E SzyjkaDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.
Shi-Jie ChenDepartment of Physics and Astronomy, University of Missouri, Columbia, Missouri 65211, USA.
Eric J StrobelDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0002-7082-2881
Nils G WalterSingle Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA.ORCID 0000-0002-7301-1275

Funding

Center for Structural Biology of HIV RNAU54AI170660 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALICE TELESNITSKY · 2022 to 2026
$32.1M
PREDOCTORAL TRAINING IN GENETICST32GM007544 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MORAN, JOHN V. · 1985 to 2022
$11.3M
The RNA nanomachines of the gene expression machinery dissected at the single molecule levelR35GM131922 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NILS G WALTER · 2019 to 2026
$6.9M
New methods for computational modeling of RNA structuresR35GM134919 · NIGMS · UNIVERSITY OF MISSOURI-COLUMBIA · PI SHI-JIE CHEN · 2020 to 2026
$3.3M
Michigan Predoctoral Training in GeneticsT32GM149391 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI JOHN V. MORAN · 2024 to 2026
$2.2M
Mechanisms of cotranscriptional RNA structure formationR35GM147137 · NIGMS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Eric J Strobel · 2022 to 2026
$2.0M
NIAID NIH HHS U54 AI170660NIGMS NIH HHS R35 GM131922NIGMS NIH HHS R35 GM134919NIGMS NIH HHS R35 GM147137NIGMS NIH HHS T32 GM007544NIGMS NIH HHS T32 GM149391
6 · The paper itself

Abstract

Riboswitches are non-coding RNA motifs that regulate gene expression in response to ligand binding. The glycine tandem riboswitch (GTR) is notable because it comprises two distinct glycine aptamers that interact extensively. These inter-aptamer contacts drive conformational changes in the downstream expression platform to control gene expression. Despite extensive studies, the role of glycine and RNA folding pathways in co-transcriptional regulation remains unclear. Here, we integrate single-molecule kinetic analysis, co-transcriptional RNA structure probing, and computational modeling to reveal that the GTR processes multiple molecular inputs sequentially, guided by polymerase pausing. Our findings elucidate its stepwise 5'-to-3' folding pathway and demonstrate how sequential glycine binding to each aptamer, K

Indexed as

coarse-grained molecular dynamics simulations and modelingCo-transcriptional RNA foldinghigh-throughput RNA structure probingriboswitchsingle-molecule fluorescence microscopy

Identifiers

PMID40501894
PMCPMC12154632

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.