Evidence map›Paper›PMID 40501878›Full record

ArticlebioRxiv : the preprint server for biology2025

In-cell Proteomics Enables High-Resolution Spatial and Temporal Mapping of Early

Jian Sun, Xiaolu Xu, Shuo Wei, Yanbao Yu

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Jian SunDepartment of Biological Sciences, University of Delaware, 105 The Green, Newark, Delaware, 19716 (USA).
Xiaolu XuDepartment of Biological Sciences, University of Delaware, 105 The Green, Newark, Delaware, 19716 (USA).
Shuo WeiDepartment of Biological Sciences, University of Delaware, 105 The Green, Newark, Delaware, 19716 (USA).
Yanbao YuDepartment of Chemistry and Biochemistry, University of Delaware, 163 The Green, Newark, Delaware, 19716 (USA).ORCID 0000-0003-2994-1974

Funding

This renovation project will create over 1455 sq. ft. of state- of-the-art reseaP20GM104316 · NIGMS · UNIVERSITY OF DELAWARE · PI FOX, JOSEPH M · 2014 to 2024
$26.8M
Mechanisms of Neural Crest Induction and Craniofacial DisordersR01DE029802 · NIDCR · UNIVERSITY OF DELAWARE · PI WEI, SHUO · 2020 to 2024
$1.8M
NIDCR NIH HHS R01 DE029802NIGMS NIH HHS P20 GM104316
6 · The paper itself

Abstract

Early embryonic development requires tightly regulated molecular programs to coordinate cell division, fate specification, and spatial patterning. While transcriptomic profiling is widely performed, proteomic analyses of early vertebrate embryos remain limited due to technical challenges in embryonic sample preparation. Here, we propose an "in-cell proteomics" approach, which bypasses cell lysis and yolk depletion, processes individual embryos directly in functionalized filter devices, and generates liquid chromatography-mass spectrometry (LC-MS)-friendly samples in an extremely robust and streamlined manner. Combined with a single-shot data-independent acquisition (DIA) MS workflow, this approach enabled us to consistently quantify ~6,200 proteins from a single

Indexed as

animal–vegetal axisE4technologyin-cell proteomicson-filter in-cell (OFIC) digestionSpatiotemporal proteomicsXenopus tropicalis

Identifiers

PMID40501878
PMCPMC12154854

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.