Evidence map›Paper›PMID 40501796›Full record

ArticlebioRxiv : the preprint server for biology2025

Ultrafast endocytosis in mouse cortical inhibitory synapses.

Chelsy R Eddings, Shigeki Watanabe

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chelsy R EddingsDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.ORCID 0000-0003-3003-8379
Shigeki WatanabeDepartment of Cell Biology, The Johns Hopkins University, Baltimore MD, 21205, USA.ORCID 0000-0001-7580-8141

Funding

Ultrafast membrane trafficking at synapsesR35NS132153 · NINDS · JOHNS HOPKINS UNIVERSITY · PI Shigeki Watanabe · 2023 to 2026
$4.1M
CRCNS: Biophysical modeling of axonal morphology and functionR01MH139350 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Padmini Rangamani · 2024 to 2026
$1.1M
Request for Leica High-pressure Freezer and Automated Freeze-substitution SystemS10RR026445 · NCRR · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2010 to 2010
$273k
NCRR NIH HHS S10 RR026445NIMH NIH HHS R01 MH139350NINDS NIH HHS R35 NS132153
6 · The paper itself

Abstract

Neural circuitry depends on excitatory and inhibitory regulation of activity to yield functional outputs. However, examinations of synaptic vesicle recycling have focused heavily on excitatory synapses, leaving many questions unanswered for inhibitory synapse dynamics. Here we show that both excitatory and inhibitory cortical synapses contain depots of a protein essential for ultrafast endocytosis, Dynamin 1xA, at a region immediately next to the active zone where ultrafast endocytosis takes place. Using zap-and-freeze time-resolved electron microscopy in mouse acute cortical slices, we observe uncoated pits reminiscent of ultrafast endocytic intermediates appearing post-stimulus at putative inhibitory synapses. These findings suggest that excitatory and inhibitory synapses may perform similar modes of endocytosis.

Indexed as

cortexDynamin 1xAhigh-pressure freezinginhibitory synapsestimulated emission depletion microscopysynaptic transmissionsynaptic vesicle endocytosistime-resolved electron microscopyultrafast endocytosiszap-and-freeze

Identifiers

PMID40501796
PMCPMC12157488

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.