Evidence map›Paper›PMID 40501792›Full record

ArticlebioRxiv : the preprint server for biology2025

VerteBrain reveals novel neural and non-neural protein assemblies conserved across vertebrate evolution.

Vy Dang, Brittney Voigt, David Yang, Gabriel Hoogerbrugge, Muyoung Lee, Rachael M Cox, Ophelia Papoulas, Claire D McWhite, Raksha Pradeep, Janelle C Leggere and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Vy DangDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0002-8754-4064
Brittney VoigtDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0003-1255-0141
David YangUniversity of California, Berkeley, Center for Computational Biology, Berkeley, California 94720, USA.
Gabriel HoogerbruggeDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0001-7617-3168
Muyoung LeeDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0002-4913-6289
Rachael M CoxDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0001-5407-1101
Ophelia PapoulasDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0002-6370-0616
Claire D McWhiteDepartment of Molecular and Cellular Biology, The University of Arizona, Tucson, Arizona 85721, USA.ORCID 0000-0001-7346-3047
Raksha PradeepDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Janelle C LeggereDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0002-5225-6046
Benjamin A NeelyChemical Sciences Division, National Institute of Standards and Technology, NIST, Charleston, South Carolina 29412, USA.ORCID 0000-0001-6120-7695
Ryan S GrayDepartment of Nutrition and Pediatrics, Dell Pediatric Research Institute, The University of Texas at Austin, Dell Medical School, Austin, TX 78712, USA.ORCID 0000-0001-9668-6497
Edward M MarcotteDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0001-8808-180X

Funding

Proteomics and model organism humanization to decode human geneticsR35GM122480 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE · 2017 to 2026
$5.4M
Uncovering the role of inppl1a in notochord vacuolation and the development of a straight body axis.F31HD114419 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI VOIGT, BRITTNEY · 2023 to 2025
$105k
NICHD NIH HHS F31 HD114419NIGMS NIH HHS R35 GM122480
6 · The paper itself

Abstract

Protein-protein interactions underlie core brain functions, including neurotransmitter release, receptor activation, and intracellular signaling essential for learning, memory, and cognition. Here, we systematically map conserved brain protein interactions across five vertebrate species-rabbit, chicken, dolphin, pig, and mouse-using co-fractionation and immunoprecipitation mass spectrometry. From 2,197 biochemical fractions, we identify over 81,000 high-confidence interactions among 6,108 conserved proteins. This interaction map (VerteBrain) reveals both regulatory and structural complexes, including extensive synaptonemal protein associations likely involved in inter-neuronal coordination. Conservation across species underscores essential roles in neuronal and glial function, as well as in additional tissues for more widely expressed complexes. The VerteBrain dataset uncovers candidate disease mechanisms, including roles for ARHGEF1 in short stature syndromes, synaptic vesicle trafficking complexes in epilepsy, and RELCH in congenital deafness. VerteBrain provides a publicly accessible framework for investigating brain protein interactions and their relevance to human neurological disorders.

Indexed as

co-fractionation mass spectrometrycomparative proteomicsHigh-throughputneuroproteomicsprotein-protein interactions

Identifiers

PMID40501792
PMCPMC12154601

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.