Evidence map›Paper›PMID 40501624›Full record

ArticlebioRxiv : the preprint server for biology2025

Multimodal retinal imaging by visible light optical coherence tomography and phosphorescence lifetime ophthalmoscopy in the mouse eye.

Stephanie Nolen, Zhongqiang Li, Jingyu Wang, Mirna El Khatib, Sergei Vinogradov, Ji Yi

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Stephanie NolenDepartment of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21231.ORCID 0000-0002-0151-5669
Zhongqiang LiDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, MD 21231.ORCID 0000-0001-6962-5146
Jingyu WangDepartment of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, MD 21231.
Mirna El KhatibDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Sergei VinogradovDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Ji YiDepartment of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21231.ORCID 0000-0001-6583-7192

Funding

A novel method for volumetric oxygen mapping in living retinaR01EY032163 · NEI · JOHNS HOPKINS UNIVERSITY · PI YI, JI · 2021 to 2024
$2.0M
Mesoscopic microscopy for ultra-high speed and large-scale volumetric brain imagingR01EB034272 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 2023 to 2026
$1.9M
Advancing visible light optical coherence tomography in glaucoma detectionR01EY034607 · NEI · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 2023 to 2026
$1.9M
Oxygen imaging by phosphorescence quenchingU24EB028941 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI VINOGRADOV, SERGEI · 2019 to 2023
$1.6M
NEI NIH HHS R01 EY032163NEI NIH HHS R01 EY034607NIBIB NIH HHS R01 EB034272NIBIB NIH HHS U24 EB028941
6 · The paper itself

Abstract

Significance: Oxygen metabolism is important to retinal disease development, but current imaging methods face challenges in resolution, throughput, and depth sectioning to spatially map microvascular oxygen. Aim: To develop a multimodal system capable of simultaneous phosphorescence lifetime imaging scanning laser ophthalmoscopy (PLIM-SLO) and visible light optical coherence tomography (VIS-OCT) to capture capillary-level oxygen partial pressure (pO Approach: C57BL/6 mice were imaged by VIS-OCT with high-definition (10 kHz raster) and Doppler (100 kHz circular) protocols. Phosphorescent probe Oxyphor 2P was retro-orbitally injected to enable intravascular PLIM-SLO imaging (200 μs pixel dwell time), while a tunable lens was used to adjust the focal depth. The extracted phosphorescence lifetimes were used for pO Results: VIS-OCT images revealed detailed anatomy and Doppler shifts, while PLIM-SLO provided capillary pO Conclusions: Detailed anatomical structures and capillary pO

Identifiers

PMID40501624
PMCPMC12154850

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.