Evidence map›Paper›PMID 40501546›Full record

ArticlebioRxiv : the preprint server for biology2025

A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging.

Hehai Jiang, Logan A Walker, Ye Li, Bin Duan, Xiaoman Niu, Jung-Chien Hsieh, Mou-Chi Cheng, Hanquan Su, Kuanwei Sheng, Jessica P Tang and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Hehai JiangSchool of Electrical and Computer Engineering, Purdue University, West Lafayette, IN.
Logan A WalkerCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.ORCID 0000-0002-5378-3315
Ye LiCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Bin DuanCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Xiaoman NiuCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Jung-Chien HsiehMichigan Neuroscience Program, University of Michigan, Ann Arbor, MI.
Mou-Chi ChengCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Hanquan SuWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Kuanwei ShengWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Jessica P TangCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Kenneth AthukoralaCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Stacey GreeneCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.
Ruby PanNew Trier High School, Winnetka, IL.
Arya ParlapalliWashtenaw International High School, Ypsilanti, MI.
Peng YinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Meng CuiSchool of Electrical and Computer Engineering, Purdue University, West Lafayette, IN.
Dawen CaiCell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI.ORCID 0000-0003-4471-2061

Funding

Development of a scalable strategy for reconstructing cell-type determined connectome of the mammalian brainRF1MH124611 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CAI, DAWEN, CUI, MENG · 2020 to 2020
$3.9M
Integrative labeling, imaging, and reconstruction tools for high-throughput inhibitory microconnectivity analysis in the mouse brainRF1MH123402 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CAI, DAWEN, YAN, YAN · 2020 to 2020
$3.1M
Continuous development of nTracer2 and its deployment at NIH image repositoriesRF1MH133764 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CAI, DAWEN, YAN, YAN · 2023 to 2023
$2.5M
NIMH NIH HHS RF1 MH123402NIMH NIH HHS RF1 MH124611NIMH NIH HHS RF1 MH133764
6 · The paper itself

Abstract

Recent advances in high throughput optical microscopy have achieved whole-organism scale imaging at diffraction-limited resolutions. Current microscopes, however, require making compromises between achieving the optimal resolution, imaging depth, multispectral capability, and data throughput due to limitations in optical design and data stream handling. We have created a parallel-line scanning confocal microscope (plSCM) which provides 1.1 Gigavoxels/second high speed imaging while achieving an optical resolution of ~180 × 220 × 650 nm with 2 millimeters of imaging depth in 3 simultaneous spectral channels. To handle such a massive imaging data stream, we have engineered a scalable network-distributed image acquisition/processing framework (SNDiF), which allows continuous capture, real-time processing, and cluster storage of petabyte-scale single image datasets in days. Together, our parallelly distributed microscope and software system presents a general solution for large-scale high-throughput and high-resolution multicolor imaging which can operate for days at a time.

Identifiers

PMID40501546
PMCPMC12157702

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.