Evidence map›Paper›PMID 40501453›Full record

ArticlePNAS nexus2025

Division of labor within polymerase theta in repair of CRISPR-induced DNA breaks in

Lycka Kamoen, Dieuwertje A de Bruin, Lejon E M Kralemann, Kim Roos, Mandy M D A Wildhagen, Robin van Schendel, Paul J J Hooykaas, Sylvia de Pater, Marcel Tijsterman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lycka KamoenDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0000-0003-3682-7037
Dieuwertje A de BruinDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0009-0009-8697-3498
Lejon E M KralemannDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0000-0002-8154-9315
Kim RoosDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0009-0007-9587-6812
Mandy M D A WildhagenDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.
Robin van SchendelDepartment of Human Genetics, Leiden University Medical Center, Einthovenweg 20, Leiden 2300 RC, The Netherlands.ORCID https://orcid.org/0000-0001-7068-0679
Paul J J HooykaasDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0000-0002-9736-6927
Sylvia de PaterDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0000-0003-2626-3661
Marcel TijstermanDepartment of Plant Sciences, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.ORCID https://orcid.org/0000-0001-8465-9002

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To develop efficient strategies for precise mutagenesis in plants, it is crucial to characterize the mechanisms involved in the repair of CRISPR-induced double strand breaks (DSBs). Polymerase theta (Polθ)-mediated end joining (TMEJ) and classical nonhomologous end joining are key pathways that generate a wide array of mutations during DSB repair. To direct repair towards more predictable outcomes, we examined the impact of direct repeats flanking DSBs, which may trigger extended microhomology-mediated end joining (eMMEJ). Unexpectedly, we found that eMMEJ in

Indexed as

Arabidopsis thalianaCRISPR/Casextended microhomology-mediated end joiningnonhomologous end joiningpolymerase theta

Identifiers

PMID40501453
PMCPMC12153051

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.