ReviewNature protocols2025
Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles.
Review in Nature protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Leveraging nanoparticle protein corona to advance plasma proteome profiling.Nature communications · 2026Review
- Influence of protein aggregates, extracellular vesicles, and lipoprotein fusion on ionizable lipid nanoparticles protein corona analysis.Nature communications · 2026Review
- The protein corona at the nano-bio interface: the need for standardized methodology and opportunities for neurodegenerative disease intervention.RSC advances · 2026Review
- Receptor discovery for nanomaterial soft and hard coronas via a biosensor-based Fishing strategy.Nature protocols · 2026Review
- Advancing nanomedicine with machine learning: predicting protein corona and nano-bio interactions.Nanomedicine (London, England) · 2026Review
- Mass Spectrometry Proteomics of the Nanoparticle Corona Is Highly Dependent on Sample Preparation Protocol.Proteomics · 2026Article
- Ligand-Mediated Proteome Remodeling Shapes Nanoparticle Protein Corona Composition for Deep Plasma Profiling.Research square · 2026Article
- Systematic evaluation of blood contamination in nanoparticle-based plasma proteomics.EMBO molecular medicine · 2026Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate and robust proteomics characterization of the protein corona is crucial for both diagnostic and therapeutic applications of nanomedicine technologies. This Perspective focuses on mass spectrometry-based characterization of the protein corona, emphasizing its role in identifying and quantifying the proteins associated with it. The discussion encompasses three major steps: protein corona formation, protein corona isolation and mass spectrometry-based characterization. Each step presents unique challenges and interdependencies; flaws and a lack of essential process/protocol information in initial steps can lead to erroneous outcomes in proteomics characterization. This Perspective also highlights the importance of standardized protocols, discusses the advantages and shortcomings of current methodologies and proposes strategies to mitigate issues such as protein corona impurities and data reproducibility. By addressing these challenges, both academic and industrial nanomedicine and proteomics communities can enhance the reliability and accuracy of protein corona studies, thereby advancing the fields of nanomedicine and proteomics.
Indexed as
Identifiers
40500335What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.