ArticleThe Plant journal : for cell and molecular biology2025
Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.).
Article in The Plant journal : for cell and molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- The mechanism of newly differentiated leaves of kale turning white in late autumn.Journal of integrative plant biology · 2026Article
- A Modified Cas9 Scaffold Allows Extension of the Virus-Induced Gene Editing Technology to the Large Potyvirus Genus.Plant biotechnology journal · 2026Article
- Developing a mobile RNA delivery system for grafting trait improvement.EMBO reports · 2026Article
- Efficient CRISPR-Cas9 delivery and transgene-free multiplex genome editing in plants using cymbidium mosaic virus-derived vectors.The Plant journal : for cell and molecular biology · 2026Article
- Virus-induced transgene- and tissue culture-free heritable genome editing in tomato.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- Virus-induced genome editing enables functional genomics across diverse plant species.Molecules and cells · 2026Review
- PCdb: A comprehensive plant genome-editing database integrating sgRNA efficiency, off-target predictions, and epigenomic landscapes.Plant communications · 2026Article
- Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.The Plant journal : for cell and molecular biology · 2026Article
- High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB.Nature plants · 2026Article
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Mobile RNAs as systemic signaling beyond boundaries for plant stress resistance.Frontiers in plant science · 2025Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Genome editing using the CRISPR/Cas system enables rapid and efficient plant breeding by directly introducing desired traits into elite lines within a short time frame. However, challenges associated with conventional Agrobacterium tumefaciens-mediated transformation and regeneration have limited gene editing in pepper (Capsicum annuum L.). In this study, we applied and optimized a virus-induced gene editing (VIGE) system to overcome these limitations. We inoculated transgenic pepper seedlings already expressing Cas9 with vectors based on tobacco rattle virus 2 (TRV2) expressing single guide RNAs (sgRNAs) targeting Phytoene desaturase (PDS); shoots regenerated from inoculated cotyledons displayed photobleaching phenotypes. To promote sgRNA mobility and maintain its integrity, we modified the pTRV2-sgRNA vector by incorporating a self-cleaving hammerhead ribozyme (HH) sequence to produce an intact sgRNA fused to part of the mobile RNA of FLOWERING LOCUS T. Additionally, we tested alternative mobile elements, such as tRNA
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