Evidence map›Paper›PMID 40498336›Full record

ArticleTransgenic research2025

Mutation in soybean Lox-2 PLAT/LH2 domain through CRISPR/Cas9 reduces seed lipoxygenase activity: responsible for undesirable flavour.

Ekta Patel, Piyali Das, Somak Hazra, Manveer Sharma, Gautam Chhabra, Balwinder Singh Gill, Sucheta Sharma, Ajinder Kaur, Deepak Singla, Jagdeep Singh Sandhu

Abstract read
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In one paragraph

Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ekta PatelSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Piyali DasSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Somak HazraSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Manveer SharmaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Gautam ChhabraSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Balwinder Singh GillDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141 004, India.
Sucheta SharmaDepartment of Biochemistry, Punjab Agricultural University, Ludhiana, 141 004, India.
Ajinder KaurSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Deepak SinglaSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India.
Jagdeep Singh SandhuSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India. js_sandhu@pau.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Soybean, a protein and oil rich legume is primarily used as livestock feed and to a lesser extent for human consumption due to undesirable flavour in the seeds caused by L-2 isozyme of lipoxygenase. Herein, soybean with reduced isozyme activity was developed through CRISPR/Cas9 targeted mutation in L-2 encoding Lox-2 gene. sgRNA designed from PLAT/LH2 domain in second exon of Lox-2 (Lox-2 E2) was validated by in vitro cleavage assay; inserted in CRISPR/Cas9 binary vector and used for genetic transformation of SL1074 cultivar hypocotyl segments. A total of 12 T

Indexed as

CRISPR-Cas SystemsGlycine maxLipoxygenasePlant ProteinsSeedsMutationPlants, Genetically ModifiedLipoxygenasePlant ProteinsBase substitutionGenome editingGlycine max L.Isozyme activityStructural modelling

Identifiers

PMID40498336
PMCPMC12158852

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.