Evidence map›Paper›PMID 40498313›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Exploring the O-glycomic Degradome Using Natural Mucin Libraries.

Marthe Tofthagen, Chunsheng Jin, Piyush Patel, Fei Sjöberg, Ana S Luis, Marissa L Maciej-Hulme, Niclas G Karlsson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Marthe TofthagenFaculty of Health Sciences, Department of Life Sciences and Health, Oslo Metropolitan University, Oslo, Norway.
Chunsheng JinProteomics Core Facility at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Piyush PatelDivision of Clinical Cancer Epidemiology, Department of Oncology, Institute of Clinical Sciences, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Fei SjöbergDivision of Clinical Cancer Epidemiology, Department of Oncology, Institute of Clinical Sciences, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Ana S LuisDepartment of Medical Biochemistry, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Marissa L Maciej-HulmeFaculty of Health Sciences, Department of Life Sciences and Health, Oslo Metropolitan University, Oslo, Norway.
Niclas G KarlssonFaculty of Health Sciences, Department of Life Sciences and Health, Oslo Metropolitan University, Oslo, Norway. niclaska@oslomet.no.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The interactions between intestinal mucins and the gut microbiota significantly influence digestive processes, metabolic activities, and pathogen resistance in the gastrointestinal tract. To explore this dynamic relationship, this chapter describes a workflow for studying the degradation of mucin oligosaccharides from isolated mucins. The approach involves purifying mucins from intestinal tissues and then subjecting them to a mixture of fecal glycosidases originating from commensal bacteria. The remaining oligosaccharides attached to the mucins after glycosidase treatment are released and then characterized and quantified using liquid chromatography-mass spectrometry (LC-MS)

Indexed as

GlycomicsMucinsAnimalsChromatography, LiquidFecesGastrointestinal MicrobiomeGlycoside HydrolasesHumansIntestinal MucosaOligosaccharidesPolysaccharidesGlycoside HydrolasesMucinsOligosaccharidesPolysaccharidesChromatographyDot blottingExoglycosidase assayLC-MSMass spectrometryMicrobiotaMucin degradationMucinsO-glycosylation

Identifiers

PMID40498313

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.