Profiling of Anti-glycan IgA and IgG in Oral Fluid Using a Glycan Microarray.

Nadezhda V Shilova, Svetlana M Polyakova, Marina V Lavrenteva, Nicolai V Bovin

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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4 authors.

Nadezhda V ShilovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Svetlana M PolyakovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Marina V LavrentevaBiotechnology Department, MIREA-Russian Technological University, Moscow, Russia.

Nicolai V BovinShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Secretory immunoglobulins (sIg), primarily IgA, control the microflora of the mucous membrane, protecting it from pathogens, as well as promoting its colonization by commensals. Knowledge of the specificity of sIgA (as well as sIgG) is necessary to understand their functions. We present here a protocol for using printed glycan array to profile the glycan specificity of sIg in oral fluid, with particular attention to sample preparation of this difficult-to-analyze material. The list of glycans in the array includes oligosaccharides typical of mucin O-chains, as well as bacterial polysaccharides, which are another full-fledged "player" in the sIg-mucin-microbiota system.

Indexed as

Immunoglobulin AImmunoglobulin A, SecretoryImmunoglobulin GMicroarray AnalysisPolysaccharidesSalivaHumansMucinsImmunoglobulin AImmunoglobulin A, SecretoryImmunoglobulin GMucinsPolysaccharidesAnti-glycan antibodiesGlycan microarrayMicrobiotaMucinsMucosal immunityOral fluidPGASecretory IgA

Identifiers

PMID40498306

DOI10.1007/978-1-0716-2148-6_5

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