Evidence map›Paper›PMID 40498296›Full record

ArticleBiotechnology journal2025

Enhanced Growth of Bone Marrow-Derived Mesenchymal Stem Cells on the Microcarriers Tethered With Engineered Basic Fibroblast Growth Factor.

Shiina Pasanen, Archana Mootha, Isao Hirata, Kotaro Tanimoto, Koichi Kato

Erratum issuedAbstract read
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In one paragraph

Article in Biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Shiina PasanenDepartment of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Archana MoothaDepartment of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Isao HirataDepartment of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Kotaro TanimotoDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Koichi KatoDepartment of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.ORCID https://orcid.org/0000-0002-0587-3527

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microcarrier-based bioreactors have been studied extensively for the expansion of human mesenchymal stem cells (hMSCs) to be used in regenerative therapy. To maximize the expansion efficiency, we developed microcarriers that tethered basic fibroblast growth factor (bFGF) on their surfaces. Using recombinant DNA technologies, bFGF was fused with a collagen-binding peptide (CBP) and bound to microcarriers that had been coated with type I collagen. In this study, we examined two types of CBP: a 28-mer peptide derived from human osteopontin (CBP1) and an 11-mer peptide derived from human decorin (CBP2). The chimeric proteins, CBP1-bFGF and CBP2-bFGF, were prepared using a bacterial expression system. Their structures were analyzed by circular dichroism spectroscopy and computer-based structure prediction, and their biological functions were assessed in hMSC cultures. Based on the results of these experiments, refolding conditions were optimized to increase the yields of soluble and bioactive CBP1-bFGF and CBP2-bFGF. Microcarriers that were tethered with CBP1-bFGF and CBP2-bFGF promoted hMSC growth without considerably altering their surface marker expression and osteogenic differentiation ability. These findings highlighted the potential of bFGF-tethered microcarriers as essential bioreactor components for hMSC expansion.

Indexed as

Bone Marrow CellsFibroblast Growth Factor 2Mesenchymal Stem CellsBioreactorsCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedDecorinHumansOsteogenesisOsteopontinRecombinant Fusion ProteinsDecorinFibroblast Growth Factor 2OsteopontinRecombinant Fusion Proteinsbioreactorcell growthgrowth factormicrocarrierregenerative medicinestem cell

Identifiers

PMID40498296
PMCPMC12154169

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.