Evidence map›Paper›PMID 40498201›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Protocols for Metabolomic Analyses to Assess Changes in Central Carbon Metabolism from Neural Tissue.

Mai Noureldein, Darcy Cochran, Fatema Bhinderwala, Shulei Lei, Jade Woods, Jordan Rose, Darrell D Marshall, Eli Riekeberg, Aline De Lima Leite, Martha Morton and 3 more

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mai Noureldein *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Darcy Cochran *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Fatema Bhinderwala *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Shulei Lei *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Jade Woods *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Jordan RoseSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Darrell D MarshallDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Eli RiekebergDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Aline De Lima LeiteDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Martha MortonDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Eric D DoddsDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Rodrigo FrancoSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Robert PowersDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA. rpowers3@unl.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Metabolomics has been successfully applied to study neurological and neurodegenerative disorders, including Parkinson's disease for the following purposes: (1) identifying potential biomarkers of onset and disease progression; (2) identifying novel mechanisms of disease progression; and (3) assessing treatment prognosis and outcome. Reproducible and efficient extraction of metabolites is imperative to the success of any metabolomics investigation. Unlike other OMICS techniques, the composition of the metabolome can be negatively impacted by the preparation, processing, and handling of these samples. The proper choice of data collection, preprocessing, and processing protocols are similarly important to the design of an effective metabolomics experiment. Likewise, correctly applying univariate and multivariate statistical methods is essential for providing biologically relevant insights. In this chapter, we have outlined a detailed metabolomics workflow that addresses these issues. A step-by-step protocol from preparing neuronal cells and metabolomic tissue samples to their metabolic analyses using nuclear magnetic resonance, mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), and chemometrics is presented.

Indexed as

CarbonMetabolomicsNerve TissueNeuronsAnimalsHumansMagnetic Resonance SpectroscopyMetabolomeSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCarbonChemometricsMALDI imagingMass SpectrometryMetabolomicsNeurodegenerationNMRParkinson’s disease

Identifiers

PMID40498201

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.