Evidence map›Paper›PMID 40498197›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Revealing the Metabolome: Metabolite Workflow for Retinal Ganglion Cells.

Alejandra Hernandez, Sofia Yarosh, Amr Almobayed, Sanjoy K Bhattacharya

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alejandra HernandezBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Sofia YaroshBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Amr AlmobayedBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Sanjoy K BhattacharyaBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA. SBhattacharya@med.miami.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this chapter, we outline the preparation and isolation of retinal ganglion cells (RGCs) from postnatal mice. This includes a metabolite extraction method using 1:1 methanol/water from 6 or 12-well plate dishes. We provide liquid chromatography-mass spectrometry (LC-MS) parameters using a QExactive High Resolution Orbitrap Mass Spectrometer and HILIC column. The software used for analysis includes Compound Discoverer and MetaboAnalyst. Through this metabolic workflow, this protocol provides a biomarker discovery, allowing for future experimentation to treat and cure ocular neuropathies.

Indexed as

MetabolomeMetabolomicsRetinal Ganglion CellsAnimalsBiomarkersChromatography, LiquidMass SpectrometryMiceSoftwareWorkflowBiomarkersAxon regenerationCompound discovererExtractionMass spectrometryMetaboAnalystMetabolomic profilingMetabolomicsRetinal ganglion cells

Identifiers

PMID40498197

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.