Evidence map›Paper›PMID 40498187›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Spatial Atmospheric Pressure MALDI HRMS Analysis of Histological Sections of FFPE Samples Reveals a Unique Subset of the Metabolome.

Gilles Frache, David C Muddiman, Simona Ospitalieri, Caleigh R O'Connor, Dietmar R Thal, Peter D E M Verhaert

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Gilles FracheLuxembourg Institute for Science and Technology, Belvaux, Luxembourg.
David C MuddimanDepartment of Chemistry, North Carolina State University, Raleigh, NC, USA.
Simona OspitalieriDepartment of Imaging and Pathology, Faculty of Medicine, University of Leuven & Department of Pathology, University Hospitals Leuven, Leuven, Belgium.
Caleigh R O'ConnorMassTech Inc., Columbia, MD, USA.
Dietmar R ThalDepartment of Imaging and Pathology, Faculty of Medicine, University of Leuven & Department of Pathology, University Hospitals Leuven, Leuven, Belgium.
Peter D E M VerhaertDepartment of Imaging and Pathology, Faculty of Medicine, University of Leuven & Department of Pathology, University Hospitals Leuven, Leuven, Belgium. peter.verhaert@proteoformix.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a method that allows high-resolution mass spectrometry (HRMS) imaging of metabolites in tissue sections from formaldehyde-fixed, paraffin-embedded (FFPE) biobanks. This top-down variant of MS imaging expands the molecular scope of mass spectrometry histochemistry (MSHC) from peptidomics to metabolomics. The method makes the vast archives of FFPE biobanks accessible for MSHC-based biomarker discovery research of not only small endogenous peptides but also (a subset of) metabolites. FFPE biobank tissues include well-documented clinical samples representing diseases with a high medical need and often presently not clinically diagnosable and/or curable.Our protocol starts with FFPE tissue sections prepared from samples procured from biobanks. We describe how to remove paraffin and coat the section with MALDI matrix while maximally reducing analyte delocalization or washout. We detail appropriate programming of the MSHC data acquisition and illustrate a way to process MSHC data (including conversion to the generic imzML format) and browse MSHC datasets. Finally, we show options to present the data in the form of annotated MSHC images.

Indexed as

MetabolomeMetabolomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTissue FixationAtmospheric PressureFormaldehydeHumansParaffin EmbeddingFormaldehydeAtmospheric pressure MALDIFormaldehyde-fixed paraffin-embedded tissuesMass spectrometry histochemistryOrbitrap HRMSTop-down mass spectrometry imaging

Identifiers

PMID40498187

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.