Evidence map›Paper›PMID 40498159›Full record

ArticleArchives of microbiology2025

Cnb proteins affect the intracellular 2-MIB retention in heterologous gene expression systems.

Kaushalya Dayarathne, Kimie Atsuzawa, Toshiki Ishikawa, Satoru Watanabe, Yasuko Kaneko, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kaushalya DayarathneGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.ORCID https://orcid.org/0000-0001-6090-9353
Kimie AtsuzawaComprehensive Analysis Center for Science, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.ORCID https://orcid.org/0000-0002-4431-4795
Toshiki IshikawaGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.ORCID https://orcid.org/0000-0001-8083-6542
Satoru WatanabeDepartment of Bioscience, Tokyo University of Agriculture, 1-chōme-1-1 Sakuragaoka, Setagaya-city, Tokyo, 156- 8502, Japan.ORCID https://orcid.org/0000-0001-7456-5053
Yasuko KanekoDepartment of Natural Science, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama city, 338- 8570, Saitama, Japan.ORCID https://orcid.org/0000-0003-4876-2859
Aikeranmu KadeerGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.
Masatoshi YamaguchiGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.ORCID https://orcid.org/0000-0002-1396-5376
Maki Kawai-YamadaGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan. mkawai@mail.saitama-u.ac.jp.ORCID https://orcid.org/0000-0002-8732-052X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

2-methylisoborneol (2-MIB) is one of the main cyanobacterial metabolites responsible for unpleasant taste and odor in drinking water. 2-MIB biosynthesis involves a two-step enzymatic reaction catalyzed by enzymes encoded by the mtf and mtc genes. Two homologous genes, cnbA and cnbB, which encode cyclic nucleotide-binding proteins of unknown function are located upstream and downstream of the mtf and mtc genes, respectively. This study investigated the involvement of cnb genes of 2-MIB-producing cyanobacterium Pseudanabaena foetida var. intermedia NIES 512 in 2-MIB synthesis using heterologous gene expression systems. Transgenic Escherichia coli and Synechococcus elongatus PCC 7942 strains expressing the cnbA-mtf-mtc-cnbB gene cluster exhibited a notable intracellular 2-MIB retention which was not shown by the transgenic strains expressing only the mtf-mtc gene unit. Immunoelectron microscopy detected that the Cnb proteins are associated with unknown intracellular compartments in transgenic S. elongatus PCC 7942 cells. This study suggests that 2-MIB-related Cnb proteins are involved in the formation of intracellular compartments, which are likely associated with intracellular 2-MIB retention.

Indexed as

Bacterial ProteinsCyanobacteriaCamphanesEscherichia coliGene ExpressionMultigene FamilySynechococcus2-methylisoborneolBacterial ProteinsCamphanes2-MIB retentionCnb proteinsCyanobacteriaIntracellular compartments

Identifiers

PMID40498159
PMCPMC12159092

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.