Evidence map›Paper›PMID 40497446›Full record

ArticleeLife2025

Single-cell transcriptomes of zebrafish germline reveal progenitor types and feminization by Foxl2l.

Chen-Wei Hsu, Hao Ho, Ching-Hsin Yang, Yan-Wei Wang, Ker-Chau Li, Bon-Chu Chung

Abstract read
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chen-Wei Hsu *National Center for Biomodels, National Applied Research Laboratories, Taipei, Taiwan.ORCID https://orcid.org/0009-0002-8845-7920
Hao Ho *Institute of Statistical Science, Academia Sinica, Taipei, Taiwan.ORCID https://orcid.org/0000-0001-8911-7907
Ching-Hsin YangInstitute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Yan-Wei WangInstitute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Ker-Chau LiInstitute of Statistical Science, Academia Sinica, Taipei, Taiwan.
Bon-Chu ChungNational Center for Biomodels, National Applied Research Laboratories, Taipei, Taiwan.ORCID https://orcid.org/0000-0002-8612-0219

Funding

National Health Research Institutes NHRI- EX107-10506SINational Science and Technology Council MOST 108-2311-B-001-038-MY3National Science and Technology Council MOST 108-2311-B-001 -038 -MY3National Science and Technology Council NSTC 113-2313-B-492-001 -National Science and Technology Council NSTC 113-2313-B-492-001-
6 · The paper itself

Abstract

Zebrafish is an important organism for genetic studies, but its early germ cell types and the mechanism of sex differentiation have not been fully characterized. Here, we profiled single-cell transcriptomes and charted a developmental trajectory going from germline stem cells, through early, committed, and late progenitors, to premeiotic and meiotic cells. We showed that the transcription factor Foxl2l was expressed in the progenitor, directing progenitor differentiation toward oocytes. CRISPR-Cas9-mediated mutation of

Indexed as

Forkhead Box Protein L2Forkhead Transcription FactorsGerm CellsSex DifferentiationStem CellsTranscriptomeZebrafishZebrafish ProteinsAnimalsFemaleGene Expression Regulation, DevelopmentalMaleSingle-Cell AnalysisForkhead Box Protein L2Forkhead Transcription FactorsZebrafish Proteinsdevelopmental biologyfemale-to-male sex changefoxl2lgerm cell progenitorgermline stem cellovarysingle-cell RNAseqzebrafish

Identifiers

PMID40497446
PMCPMC12289312

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.