Evidence map›Paper›PMID 40496565›Full record

ArticleFrontiers in endocrinology2025

Single cell sequencing revealed parathyroid oxyphil cells are involved in osteoporosis under primary hyperparathyroidism.

Xinguo Zhang, Ruifeng Bai, Minjuan Li, Zhigang Li, Xian Zhao, Renwei Cao, Shen Tan, Kaiyuan Cheng, Yejun Zha, Xieyuan Jiang and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinguo ZhangDepartment of Orthopedic, Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, China.
Ruifeng BaiDepartment of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Minjuan LiDepartment of Orthopedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Zhigang LiState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, China.
Xian ZhaoDepartment of Orthopedics Trauma, Beijing Jishuitan Hospital, Peking University Fourth School, Beijing, China.
Renwei CaoDepartment of Orthopedics Trauma, Beijing Jishuitan Hospital, Peking University Fourth School, Beijing, China.
Shen TanDepartment of General Surgery, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Kaiyuan ChengDepartment of Orthopedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Yejun ZhaDepartment of Orthopedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Xieyuan JiangDepartment of Orthopedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Shuai LuDepartment of Orthopedic Trauma, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To analyze the heterogeneity of parathyroid cells between patients with primary hyperparathyroidism (PHPT) osteoporosis and PHPT non-osteoporosis patients. Methods: Resected parathyroid tissues were collected from PHPT patients of osteoporosis and non-osteoporosis. Single cell sequencing (SCS) to investigate cell types in parathyroid tissue involved in osteoporosis under PHPT. Further cell-cell interaction and communication, pseudotime trajectory analysis, sub-population analysis of parathyroid chief cells and parathyroid oxyphil cells, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional prediction analysis to confirm specific function of parathyroid cells. Results: Hallmark-IL2/STAT5 and WNT/β-catenin pathways were upregulated in parathyroid cells of osteoporosis patients. Highest interactions and cell-cell communications were enriched in parathyroid cells. Subcluster analysis disclosed overall highest 31.86% CXCL10-PCC parathyroid chief cells, but SPARCL1-OC parathyroid oxyphil cells were higher in osteoporosis patients. Pseudotime trajectory analysis displayed that parathyroid oxyphil cells were in abundance in osteoporosis patients. In total, 281 DEGs involved in kinase activity were identified in osteoporosis patients. Heatmap showed HSPA1A-OC parathyroid oxyphil cells are predominantly involved in numerous and strongest cell interactions. GO and KEGG enrichment revealed PTH, NOTCH, FGF, EGF and CD59 pathways were significantly up-regulated in all parathyroid subpopulations in osteoporosis patients. Conclusion: Single cell sequencing revealed highest number of parathyroid cells in parathyroid tissue in patients suffering with PHPT osteoporosis. Parathyroid oxyphil cells are predominantly involved in osteoporosis under PHPT.

Indexed as

Hyperparathyroidism, PrimaryOsteoporosisParathyroid GlandsSingle-Cell AnalysisAgedCell CommunicationFemaleHumansMaleMiddle Agedboneosteoporosisparathyroid oxyphil cellsprimary hyperparathyroidismsingle cell sequencing

Identifiers

PMID40496565
PMCPMC12148854

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.