Evidence map›Paper›PMID 40495228›Full record

ArticleJournal of ovarian research2025

MACS and acellular autologous non-ovarian tissue scaffolds: a promising strategy for safe and efficient follicle transplantation in hematologic cancer.

Lijun Yin, Yating Zhao, Peiwen Zhang, Tongyun Qi, Peilin Han, Luya Cai, Jun Pan, Yongyi Yang, Jie Shi, Shi Feng and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Lijun YinDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China. yinlijunwxjs@zju.edu.cn.
Yating ZhaoDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Peiwen ZhangDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Tongyun QiDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Peilin HanDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Luya CaiDepartment of Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Zhe Jiang, Wenzhou, 325035, China.
Jun PanThyroid Disease Diagnosis and Treatment Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Yongyi YangDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Jie ShiDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Shi FengDepartment of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Yinying ZouDepartment of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Kangxin HeState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Guoliang XieDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Weihua HeDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.
Xinhui ZhouDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China. 1194083@zju.edu.cn.
Jianhua QianDepartment of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China.

Funding

Joint Funds of the Zhejiang Provincial Natural Science Foundation of China No. LBY21H180002National Natural Science Foundation of China N0.82101771Zhejiang Fundamental Public Welfare Research Program LGF22H200001
6 · The paper itself

Abstract

backgroundAdvances in ovarian tissue cryopreservation offer new hope for young hematologic cancer patients. However, the risk of cancer cell reintroduction during transplantation remains a major concern, necessitating both effective tumor cell removal strategies and biocompatible scaffold development.

methodsWe characterized decellularized adipose, peritoneal, and ovarian tissue scaffolds through H&E staining, immunofluorescence, SEM, and proliferation assays. Magnetic-activated cell sorting (MACS) efficiency was evaluated for reducing hematologic malignancy contamination. Follicle function was assessed via immunofluorescence and ELISA, while RNA-seq and qPCR compared gene expression across scaffolds.

resultsSodium dodecyl sulfate (SDS) decellularization effectively preserved extracellular matrix architecture across all tissues. In lipopolysaccharide (LPS)-induced leukocytosis models, MACS significantly reduced leukocyte contamination (p < 0.0001). Comparable follicle growth and hormone production (estrogen/progesterone/inhibin) were observed across scaffolds. RNA-seq analysis identified subtle differential expression in a small subset of follicle function-related genes, while the majority of genes exhibited conserved expression patterns across scaffolds.

conclusionThe results demonstrate that MACS effectively prevents tumor cell transmission during follicle transplantation. All decellularized scaffolds exhibited high follicular biocompatibility in this animal model, with non-ovarian scaffolds emerging as promising autologous alternatives for artificial ovary engineering.

Indexed as

Hematologic NeoplasmsOvarian FollicleTissue ScaffoldsAnimalsFemaleHumansMiceOvaryTissue EngineeringTransplantation, AutologousAdipose tissueDecellularized scaffoldsExtracellular matrixFollicle transplantationMagnetic Activated Cell Sorting (MACS)Ovarian tissuePeritoneal tissuePreantral follicle growthTumor cell contamination reduction

Identifiers

PMID40495228
PMCPMC12150555

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.