ReviewCurrent opinion in structural biology2025
Quantitative analysis methods for free diffusion single-molecule FRET experiments.
Review in Current opinion in structural biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET.Cell reports methods · 2026Article
- Three-color single-molecule fluorescence resonance energy transfer to study macromolecular dynamics.Current opinion in structural biology · 2026Review
- Three-color single-molecule maximum likelihood analysis of folding and binding of diffusing molecules.Biophysical journal · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique for investigating the structure and dynamics of biomolecules. This review focuses on recent advances in quantitative methods to analyze freely diffusing molecules in smFRET. The methods include traditional approaches of analyzing FRET efficiency and advanced photon-by-photon techniques based on maximum likelihood estimation without binning photon sequences. More recently, methods explicitly accounting for molecular diffusion have been developed, addressing biases arising from variations in brightness and diffusivity among molecular states and species. Applications of these tools include studies of protein folding, DNA dynamics, and oligomerization processes of neurodegenerative proteins. These advancements expand the ability of free diffusion-based smFRET to elucidate the dynamic behavior of biomolecules on the timescales relevant to their biological processes.
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