ArticleAngewandte Chemie (International ed. in English)2025
SPLICEIT Fluorescent Sensor for Integrating Dopamine Release with Cellular Resolution.
Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- SENSIT, a Modular Single-Chain Fluorescent Integrator Platform for GPCR Ligands.ACS chemical biology · 2026Article
- Visualizing Extracellular Dopamine Dynamics in the Retina Reveals a Biphasic Reduction During Initial Myopia Development.Investigative ophthalmology & visual science · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Dopamine is a neurotransmitter essential for motor control, reward processing, and motivation through G-protein coupled receptor (GPCR) signaling. Recent GPCR-based real-time sensors allow for optical monitoring of dopamine release in behaving animals. However, there is still a need for high resolution mapping of dopamine release across large mouse brain volume for systemic studies. To fill this need, we have developed a new chimeric GPCR-based sensor to detect dopamine with a permanent green fluorescent mark through a combination of computational modeling and rational design. This new sensor, named "Single-chain Protein-based Ligand Indication through a Chimerically-Engineered Integrator Tool" (SPLICEIT), detects dopamine at cellular-resolution with high specificity and a fourfold signal-to-background ratio. We also developed a version of SPLICEIT whose signal can be normalized for varying sensor expression in cell culture and in vivo. This paves the way for further studies into dopamine release across the brain and enables the possibility of whole-brain dopamine mapping.
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Registered trials
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