Click & sea: using bioorthogonal click chemistry to visualize seaweed cell walls.

objectivesThe study of seaweed cell walls, including their metabolism and composition, is crucial to monitor and understand their adaptation to climate change. Microscopy-based techniques that facilitate studies of seaweed cell walls in situ, including staining and immunolabelling, exist but have significant limitations, including that only a few monoclonal antibodies have been developed towards seaweed cell wall components. Furthermore, not all seaweed cell wall components have been described fully. This makes in situ studies focused on the metabolism of seaweed cell walls particularly challenging. Here, we present a method for labelling seaweed cell walls by incorporating chemical reporters in muro, followed by their association with fluorophores by click chemistry.

methodsTwo different species of seaweed, representing different groups, i.e. the red seaweed Phycodrys rubens and the green seaweed Ulva spp., were selected for their abundance on the Irish coast and because they have relatively thin tissues, a feature likely to facilitate uptake and labelling using monosaccharide reporters. We selected three different activated sugars (fucose, galactose and glucose analogues) on the basis that they are major components of seaweed polysaccharides. Small sections of the seaweeds were incubated with the activated sugars, and their uptake and incorporation were visualized by attachment to the fluorescent probe AF488 and imaged using confocal microscopy.

resultsAfter incubation with the activated sugars, the seaweed cell walls, i.e. the contours of the cells and, to a lesser extent, some cell organelles, fluoresced at 517 nm (emission wavelength of AF488), suggesting that the seaweed had incorporated the activated sugars. Fiji software was used to remove non-specific fluorescence (autofluorescence of the seaweed and non-specific binding of the fluorochrome), with the final images suggesting good and specific incorporation of the fluorochrome by the seaweeds. More interestingly, the fluorescence was associated primarily with the cell walls, implying that the activated sugars were incorporated predominantly into cell wall components (most probably either polysaccharides or glycoproteins).

conclusionTo date, bioorthogonal click chemistry has not been applied to seaweeds, but it represents a useful tool for phycologists to gain a better understanding of seaweed cell wall composition and dynamics, for example, throughout the seaweed life cycle or in the face of stresses (biotic or abiotic), including those resulting from climate change.