ArticleBiotechnology journal2025
Replacement of Isoleucine and Leucine by their Keto Acids leads to increased formation of α-Hydroxy Acids in Chinese Hamster Ovary cells.
Article in Biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Impact of keto leucine and isoleucine on CHO cell central carbon metabolism and performance in fed-batch and steady-state perfusion.Frontiers in bioengineering and biotechnology · 2026Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keto isoleucine and keto leucine are bioavailable amino acid precursors in CHO cells producing biotherapeutics. Following their supplementation in a fed-batch culture with CHOK1 GS cells, quantitation of metabolites derived from the three branched-chain keto acids revealed a decrease of their decarboxylation products, but an accumulation of their α-hydroxy acids. The latter had minor effects on cell growth and productivity. L-lactate dehydrogenase was identified as the key enzyme forming valine's α-hydroxy acid.
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Registered trials
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