Evidence map›Paper›PMID 40490984›Full record

ArticleBiotechnology journal2025

Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production.

Beatriz de Oliveira Vargas, Marcelo Falsarella Carazzolle, Juliana Pimentel Galhardo, Juliana José, Brenda Cristina de Souza, Jéssica Batista de Lima Correia, Jade Ribeiro Dos Santos, Gonçalo Amarante Guimarães Pereira, Fellipe da Silveira Bezerra de de Mello

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Beatriz de Oliveira VargasDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Marcelo Falsarella CarazzolleDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Juliana Pimentel GalhardoDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Juliana JoséDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Brenda Cristina de SouzaDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Jéssica Batista de Lima CorreiaDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Jade Ribeiro Dos SantosDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.
Gonçalo Amarante Guimarães PereiraDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.ORCID https://orcid.org/0000-0003-4140-3482
Fellipe da Silveira Bezerra de de MelloDepartamento de Genética, Evolução, Microbiologia e Imunologia, UNICAMP, Campinas, São Paulo, Brazil.ORCID https://orcid.org/0000-0001-5842-1682

Funding

Fundação de Amparo à Pesquisa no Estado de São Paulo BCS: 2022/05001-4National Agency of Petroleum, Natural Gas and BiofuelsNational Council for the Improvement of Higher Education JPG: 88887.479699/2020-0National Council for the Improvement of Higher Education JRS: 142340/2020-0
6 · The paper itself

Abstract

Xylose metabolism in Saccharomyces cerevisiae remains a significant bottleneck due to the difficulty in identifying functional and efficient xylose isomerases (XI). In the present study, publicly available metagenomic and metatranscriptomic datasets of rumen microbiota from different herbivorous mammals were used to prospect novel XIs sequences. Seven putative XIs from moose, camel, cow, and sheep were cloned into a strain modified for xylose metabolism. Out of those, five XIs demonstrated activity and efficiently converted xylose into xylulose, resulting in ethanol as the final product. A XI from camel rumen microbiota exhibited a K

Indexed as

BiofuelsMetabolic EngineeringRumenSaccharomyces cerevisiaeAldose-Ketose IsomerasesAnimalsCamelusCattleEthanolFermentationMicrobiotaSheepXyloseAldose-Ketose IsomerasesBiofuelsEthanolXylosexylose isomeraseSaccharomyces cerevisiaesecond‐generation ethanolxylose isomerase

Identifiers

PMID40490984
PMCPMC12149495

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.