Evidence map›Paper›PMID 40490757›Full record

ArticleParasites & vectors2025

In vitro interaction profiles and midgut glycoconjugates of Trichophoromyia spp./Leishmania (Viannia) lainsoni.

Thais Gouvea de Morais, Yetsenia Del Valle Sánchez Uzcátegui, Chubert Bernardo Castro de Sena, Rodrigo Ribeiro Furtado, Fernando Tobias Silveira, Edilene Oliveira da Silva, Thiago Vasconcelos Dos Santos

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In one paragraph

Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Thais Gouvea de MoraisPrograma de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Yetsenia Del Valle Sánchez UzcáteguiPrograma de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Chubert Bernardo Castro de SenaLaboratório de Biologia Estrutural, Universidade Federal do Pará, Belém, Pará, Brazil.
Rodrigo Ribeiro FurtadoPrograma de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Fernando Tobias SilveiraSeção de Parasitologia, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
Edilene Oliveira da Silva *Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Thiago Vasconcelos Dos Santos *Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil. thiagovasconcelos@iec.gov.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico grant no. 314890/2021-1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Financial code 001Coordenação de Aperfeiçoamento de Pessoal de Nível Superior PDSE (44/2022; 01/2023
6 · The paper itself

Abstract

backgroundKnowledge of sand fly-Leishmania attachment determinants is pivotal for providing evidence on vector status. Considering the Amazonian transmission context of Trichophoromyia spp.-L. (Viannia) lainsoni, the present study aimed to assess in vitro interactions and detect gut glycoconjugates associated with this vector-parasite association.

methodsField-caught Trichophoromyia brachipyga and Trichophoromyia ubiquitalis were tested. Lutzomyia longipalpis reared in the laboratory was used as a control. The intestines were obtained by dissection, and the species were confirmed by morphology. Interactions for each sand fly-Leishmania association were individually performed via an in vitro incubation system. N-acetyl-D-glucosamine (GlcNAc), galactose-(β 1,3)-GalNAc (Gal/GalNAc), and N-acetyl-D-galactosamine (GalNAc) glycoconjugates of Trichophoromyia spp. were analyzed by Western blotting using corresponding peroxidase-conjugated lectins.

resultsNo difference was found between Th. ubiquitalis and Lu. longipalpis attachment with L. (V.) lainsoni, and Lu. longipalpis with Leishmania (Leishmania) infantum (control). However, Th. brachipyga-L. (V.) lainsoni attachment was weaker than that of the control. Trichophoromyia spp. were negative for residues of GlcNAc terminally exposed. Trichophoromyia ubiquitalis was positive for GalNAc and Gal/GalNAc, whereas Th. brachipyga presented only residues of GalNac terminally exposed.

conclusionsThe present study suggests that Trichophoromyia spp. sand flies, particularly Th. brachipyga, are susceptible to L. (V.) lainsoni, based on the observed vector-parasite attachment profiles and detection of GalNAc in their midguts. This supports early field data suggesting the vector status of these sand fly species.

Indexed as

GlycoconjugatesInsect VectorsLeishmaniaPsychodidaeAnimalsFemaleHost-Parasite InteractionsGlycoconjugatesAttachmentGlycoconjugatesLeishmaniaSand flyVector

Identifiers

PMID40490757
PMCPMC12150580

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.