Evidence map›Paper›PMID 40490540›Full record

ArticleCommunications biology2025

Pore formation by the CDTb component of the Clostridioides difficile binary toxin is Ca

Dinendra L Abeyawardhane, Spiridon E Sevdalis, Kaylin A Adipietro, Raquel Godoy-Ruiz, Kristen M Varney, Izza F Nawaz, Alejandro X Spittel, Daniel Hunter, Richard R Rustandi, Vitalii I Silin and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Dinendra L Abeyawardhane *Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-3002-7792
Spiridon E Sevdalis *Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0009-0009-7846-1177
Kaylin A AdipietroDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0009-0006-1484-6850
Raquel Godoy-RuizDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-4569-0781
Kristen M VarneyDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-3283-4950
Izza F NawazDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.
Alejandro X SpittelDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.
Daniel HunterDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.
Richard R RustandiMerck & Co. Inc., Kenilworth, NJ, USA.ORCID http://orcid.org/0000-0002-2287-5188
Vitalii I SilinInstitute for Bioscience and Biotechnology Research (IBBR), University of Maryland, Rockville, MD, USA.ORCID http://orcid.org/0009-0001-3276-0229
Amedee des GeorgesStructural Biology Initiative, CUNY Advanced Science Research Center, New York, NY, USA.ORCID http://orcid.org/0000-0002-9704-3781
Mary E CookDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-6298-2586
Edwin PozharskiDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA. epozharskiy@som.umaryland.edu.ORCID http://orcid.org/0000-0001-7012-5376
David J WeberDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA. dweber@som.umaryland.edu.ORCID http://orcid.org/0000-0002-8824-1110

Funding

UNIVERSITY OF MARYLAND GREENEBAUM CANCER CENTERSUPPORT GRANTP30CA134274 · NCI · UNIVERSITY OF MARYLAND BALTIMORE · PI Vu H. Duong · 2008 to 2026
$51.0M
Research Training in Gastroenterology and HepatologyT32DK067872 · NIDDK · UNIVERSITY OF MARYLAND BALTIMORE · PI RAUFMAN, JEAN-PIERRE · 2005 to 2024
$7.2M
Signal Propagation in Protein Allostery: Mechanism and EvolutionR01GM129327 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI WEBER, DAVID JOSEPH · 2019 to 2022
$1.3M
Structure-based targeting of the C. difficile toxin (CDT) from hypervirulent bacterial strainsR56AI152397 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI DES GEORGES, AMEDEE, WEBER, DAVID JOSEPH · 2021 to 2021
$522k
NCI NIH HHS P30 CA134274NIAID NIH HHS R56 AI152397NIDDK NIH HHS T32 DK067872NIGMS NIH HHS R01 GM129327
6 · The paper itself

Abstract

Clostridioides difficile infection (CDI) is one of the five most urgent bacterial threats in the United States. Furthermore, hypervirulent CDI strains express a third toxin termed the C. difficile binary toxin (CDT), and its molecular mechanism for entering host cells is not fully elucidated. Like other AB-type binary toxins, CDT enters host cells via endosomes. Here we show via surface plasmon resonance and electrochemical impedance spectroscopy that the cell-binding component of CDT, termed CDTb, binds and form pores in lipid bilayers in the absence of its enzymatic component, CDTa. This occurs upon lowering free Ca

Indexed as

ADP Ribose TransferasesBacterial ProteinsBacterial ToxinsCalciumClostridioides difficileCryoelectron MicroscopyCrystallography, X-RayLipid BilayersProtein Bindingactin-specific ADP-ribosyltransferase, ClostridiumADP Ribose TransferasesBacterial ProteinsBacterial ToxinsCalciumLipid Bilayers

Identifiers

PMID40490540
PMCPMC12149301

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.