ArticleEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society2025
Exploring the role of Serpins as a potential molecular target in intervertebral disc degeneration.
Article in European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
purposeA critical event in the disc degeneration process is the breakdown of the extra-cellular matrix regulated by proteases. These proteases are kept in check by inhibitors, most importantly SERPINs. Understanding tissue and cellular expression of SERPINs could provide more insights into their physiological roles in intervertebral disc (IVD) homeostasis.
methodsTwelve lumbar IVD NP tissues from each group- brain-dead voluntary organ donors (ND); patients with herniated discs (DH) and degenerated discs (DD) were used in this study. Label-free global proteomics approach was conducted to understand the altered patterns of SERPINs. Validation of SERPINs was performed using immunohistochemical analysis (IHC) and western blotting (WB).
resultsWe identified 21 SERPINs, among which 14 passed stringent cut-off. Comparative analysis revealed that 7 SERPINs namely SERPINA1, SERPINA3, SERPINA5, SERPINA8, SERPINC1, SERPINE2, and SERPING1 were common to all the groups. We noticed that SERPINA4, SERPIND1, SERPINF1, SERPINF2 and SERPINH1 was found common to DH and DD groups and SERPINE1 and SERPINB1 were found unique to ND and DD groups, respectively. Significantly decreased expression levels of SERPINA5 (DD, 0.010), SERPINE2 (DD, 0.028) and SERPING1 (DD, 0.000) were found in DD groups when compared with the ND group.
conclusionOur study documents the altered expression of SERPINs in IVD degeneration. The decreased expression of specific, namely SERPINA5, SERPINE2, and SERPING1, can be one of the possible causes of increased ECM degradation in IVDD. However, further studies need to explore the role of SERPINs as a potential molecular target in preventing ECM degradation.
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