ReviewFrontiers in plant science2025
A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.
Review in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Rapid Visual Detection ofJournal of fungi (Basel, Switzerland) · 2026Article
- RPA-CRISPR/Cas12a-Mediated Isothermal Amplification Technology for Visual Detection ofPlants (Basel, Switzerland) · 2026Article
- Rapid On-Site Detection ofPlants (Basel, Switzerland) · 2026Article
- Article
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- Advancements in somatic mutation detection through loop-mediated isothermal amplification (LAMP) in human tumors.Journal of applied genetics · 2026Review
- Development of a Field-Deployable Loop-Mediated Isothermal Amplification Assay for the Rapid Detection ofJournal of fungi (Basel, Switzerland) · 2026Article
- Robust design of LAMP assays for in-field detection of major bacterial vascular diseases of banana.PloS one · 2026Article
- Article
- LAMP-Based Detection ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
- Point-of-Care Diagnostic Testing for Emerging and Existing Poultry Viral Respiratory Pathogens Using Loop-Mediated Isothermal Amplification.Pathogens (Basel, Switzerland) · 2025Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and identification of plant pathogenic fungi are crucial for effective plant protection measures. In the past two decades, loop-mediated isothermal amplification (LAMP) has emerged as a simple and cost-efficient tool for plant disease diagnosis, overcoming many drawbacks of traditional and PCR-based methods. LAMP relies on efficient DNA synthesis at a constant temperature, eliminating the need for thermocycling equipment. It is typically more robust, specific, and sensitive than PCR. This literature review summarizes LAMP primer design, reaction protocol development, sensitivity and specificity testing, and result detection methods. We provide examples of how LAMP's advantages are exploited in disease diagnosis and survey its diverse applications in plant pathogenic fungi research. These applications include the detection, identification, and monitoring of plant pathogenic fungi; the replacement of culture-based methods; the detection of genetic regions associated with functional changes; and the detection of single nucleotide polymorphisms. A comprehensive list of available assays is also provided. Despite its shortcomings-including difficulties with primer design, risks of cross-contamination, and the potential for false positives-LAMP holds significant potential to gain widespread recognition and popularity in the study of plant pathogenic fungi.
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Registered trials
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