Evidence map›Paper›PMID 40487111›Full record

ArticleAnimal nutrition (Zhongguo xu mu shou yi xue hui)2025

Myotonic dystrophy-related CDC42-binding kinase alpha (MRCKα) mediates methionine- and leucine-stimulated β-casein synthesis in bovine mammary epithelial cells via targeting mTOR.

Fang Wang, Jürgen van Baal, Lu Ma, Xuejun Gao, Jan Dijkstra, Dengpan Bu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal nutrition (Zhongguo xu mu shou yi xue hui), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Fang WangState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Jürgen van BaalAnimal Nutrition Group, Wageningen University and Research, Wageningen, 6708, WD, the Netherlands.
Lu MaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Xuejun GaoCollege of Animal Science, Yangtze University, Jingzhou, 434020, China.
Jan DijkstraAnimal Nutrition Group, Wageningen University and Research, Wageningen, 6708, WD, the Netherlands.
Dengpan BuState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Amino acids (AA), including methionine (Met) and leucine (Leu), stimulate milk synthesis in bovine mammary epithelial cells (BMEC) via activation of protein kinase mechanistic target of rapamycin (mTOR). In this study, we further explored the potential role of myotonic dystrophy-related CDC42-binding kinase alpha (MRCKα), previously identified as a critical mediator of prolactin-stimulated milk synthesis in BMEC. Administering different doses (0, 0.2, 0.4, 0.6, 0.8 mM) of Met or Leu to a primary BMEC culture showed that 0.6 mM was the optimal dose for stimulating β-casein production with both AA. At this dose, Met and Leu independently evoked higher (

Indexed as

Amino acidMammary glandMilk synthesisMyotonic dystrophy-related CDC42-binding kinase alphaPhosphatidylinositol 3-kinaseProtein kinase B

Identifiers

PMID40487111
PMCPMC12143634

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.