Evidence map›Paper›PMID 40486258›Full record

ArticleJournal of arthropod-borne diseases2024

Molecular Characterization and Phylogenetic Analysis of Flea Species in Human and Livestock Residence by Targeting ITS2 Region in East Azerbaijan Province, Iran.

Mostafa Farmani, Adel Spotin, Afsaneh Dolatkhah, Saber Gholizadeh, Teimour Hazratian

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Article in Journal of arthropod-borne diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Mostafa FarmaniDepartment of Parasitology, Entomology and Medical Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Adel SpotinDepartment of Parasitology, Entomology and Medical Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Afsaneh DolatkhahDepartment of Parasitology, Entomology and Medical Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Saber GholizadehDepartment of Medical Entomology, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran.
Teimour HazratianDepartment of Parasitology, Entomology and Medical Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Fleas are blood-sucking ectoparasites with complete metamorphosis. They belong to the order Siphonaptera and can infest both humans and animals, causing dermatitis and transmitting vector-borne diseases. Despite extensive study of their classification and biology, the phylogenetic relationship between fleas in Iran is not fully understood. This research aimed to identify the flea species collected from different parts of East Azerbaijan Province in northwest Iran, using morphological, molecular, and phylogenetic analysis. Methods: From October 2019 to October 2020, we collected fleas using various methods such as hand catching for humans, brushing for dogs and cats, sticky traps for rodent burrows, light traps, and dishes with water for sheep and goats. After identifying the flea species using morphological identification keys, we extracted total genomic DNA and amplified it by targeting the ITS2 region. The PCR products were then directly sequenced to investigate the flea species. Results: In total, 1929 flea specimens were collected, revealing three genera and four species. The breakdown of the specimens is as follows: Conclusion: This study represents the first in-depth analysis in East Azerbaijan Province, highlighting the significance of considering

Indexed as

East AzerbaijanFleasIranITS2PhylogenySiphonaptera

Identifiers

PMID40486258
PMCPMC12144860

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