ArticleThe Plant cell2025
Small DNA elements can act as both insulators and silencers in plants.
Article in The Plant cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Engineering climate-resilient horticultural crops: advances in transcriptional regulation, genome editing, and synthetic networks.Horticulture research · 2026Article
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Genome-wide modelling of plant transcription factor binding captures regulatory variants associated with phenotypic traits.Nature communications · 2026Article
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Spatio-temporal regulation of recombinase expression enables efficient autoexcision of selectable marker genes in soybean and maize.BMC plant biology · 2025Article
- Chromatin insulators: Good fences that make good neighbors.The Plant cell · 2025Article
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19 authors.
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Abstract
Insulators are cis-regulatory elements that separate transcriptional units, whereas silencers are elements that repress transcription regardless of their position. In plants, these elements remain largely uncharacterized. Here, we use the massively parallel reporter assay Plant STARR-seq with short fragments of 8 large insulators to identify more than 100 fragments that block enhancer activity. The short fragments can be combined to generate more powerful insulators that abolish the capacity of the strong viral 35S enhancer to activate the 35S minimal promoter. Unexpectedly, when tested upstream of weak enhancers, these fragments act as silencers and repress transcription. Thus, these elements are capable of insulating or repressing transcription, depending on the regulatory context. We validate our findings in stable transgenic Arabidopsis thaliana, maize (Zea mays), and rice (Oryza sativa) plants. The short elements identified here should be useful building blocks for plant biotechnology.
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