Evidence map›Paper›PMID 40485304›Full record

ArticleBiotechnology progress

Optimization of multi-column chromatography for capture and polishing at high protein load.

Tiago Castanheira Silva, Madelène Isaksson, Bernt Nilsson, Michel Eppink, Marcel Ottens

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tiago Castanheira SilvaDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.ORCID 0000-0001-9399-7302
Madelène IsakssonDepartment of Chemical Engineering, Lund University, Lund, Sweden.
Bernt NilssonDepartment of Chemical Engineering, Lund University, Lund, Sweden.
Michel EppinkDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Marcel OttensDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.

Funding

Horizon 2020 Framework Programme 812909
6 · The paper itself

Abstract

Integrated Continuous Biomanufacturing reduces manufacturing costs while maintaining product quality. A key contributor to high biopharmaceutical costs, specifically monoclonal antibodies (mAbs), is chromatography. Protein A ligands are usually preferred but still expensive in the manufacturing context, and batch chromatography under-utilizes the columns' capacity, compromising productivity to maintain high yields. Continuous chromatography increases columns' Capacity Utilization (CU) without sacrificing yield or productivity. This work presents the in-silico optimization of a 3 Column Periodic Counter-current Chromatography (3C-PCC) of a capture and polishing step for mAbs from a high titer harvest (c

Indexed as

Antibodies, MonoclonalAnimalsCHO CellsCountercurrent DistributionCricetulusStaphylococcal Protein AAntibodies, MonoclonalStaphylococcal Protein Acontinuous chromatographyhigh titersintegrated continuous biomanufacturingmodelingperiodic counter‐current chromatography

Identifiers

PMID40485304
PMCPMC12531920

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.