Evidence map›Paper›PMID 40485106›Full record

ArticleMicrobial biotechnology2025

Toehold Switch-Based Approach for Engineering Acid-Tolerance Modules to Enhance Production Robustness of Industrial E. coli Strains at Low pH.

Xin Zhang, Xiaofang Yan, Peng Liu, Haozheng Huang, Zhanglin Lin, Xiaofeng Yang

Abstract read
In one paragraph

Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xin ZhangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.
Xiaofang YanSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.
Peng LiuSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.
Haozheng HuangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.ORCID https://orcid.org/0009-0003-6014-6191
Zhanglin LinSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.ORCID https://orcid.org/0000-0002-5223-7013
Xiaofeng YangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, China.ORCID https://orcid.org/0000-0002-0828-0807

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2024A1515030121National Key Research and Development Program of China 2018YFA0901000
6 · The paper itself

Abstract

Enhancing acid tolerance of industrial microorganisms is critical for improving fermentation efficiency and sustainability. This study presents a synthetic biology approach that employs toehold switch-based acid-tolerance modules to engineer acid-tolerant strains. This toehold switch-based approach enables the construction of modules consisting of a trigger block and a switch block, generating a synthetic module library of ~10

Indexed as

AcidsEscherichia coliMetabolic EngineeringFermentationGene Expression ProfilingGene Expression Regulation, BacterialHydrogen-Ion ConcentrationIndustrial MicrobiologyLysineSynthetic BiologyAcidsLysineacid tolerancelysine productionstepwise evaluationsynthetic modulestoehold switch

Identifiers

PMID40485106
PMCPMC12146417

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.