Evidence map›Paper›PMID 40484963›Full record

ArticleBMC microbiology2025

Simulated microgravity triggers a membrane adaptation to stress in E. coli REL606.

Brittney Lozzi, Lea Adepoju, Josh L Espinoza, Michael Padgen, Macarena Parra, Antonio Ricco, Sarah Castro-Wallace, Jeffrey E Barrick, Aubrie O'Rourke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Brittney LozziSpace Center Office of STEM Engagement (OSTEM) Intern Program, NASA Kennedy, Kennedy Space Center, Merritt Island, FL, 32899, USA.
Lea AdepojuSpace Center Office of STEM Engagement (OSTEM) Intern Program, NASA Kennedy, Kennedy Space Center, Merritt Island, FL, 32899, USA.
Josh L EspinozaDepartment of Environment and Sustainability, J. Craig Venter Institute, 4120 Capricorn Way, San Diego, CA, 92037, USA.
Michael PadgenNASA Ames Research Center, Moffett Field, CA, USA.
Macarena ParraNASA Ames Research Center, Moffett Field, CA, USA.
Antonio RiccoNASA Ames Research Center, Moffett Field, CA, USA.
Sarah Castro-WallaceBiomedical Research and Environmental Sciences Division, NASA Johnson Space Center, Houston, TX, USA.
Jeffrey E BarrickDepartment of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, 2500 Speedway A5000, Austin, TX, 78751, USA.
Aubrie O'RourkeNASA Exploration Research and Technology, Kennedy Space Center, Merritt Island, FL, 32899, USA. Aubrie.e.orourke@nasa.gov.

Funding

National Science Foundation DEB-1951307
6 · The paper itself

Abstract

Investigating the evolution of Escherichia coli in microgravity offers valuable insights into microbial adaptation to extreme environments. Here the effects of simulated microgravity (SµG) on gene expression and genome evolution of E. coli REL606, a strain evolved terrestrially for 35 years, is explored. The transcriptomic changes for glucose-limited and glucose-replete conditions over 24 h illustrate that SµG increased the expression of genes involved in stress response, biofilm, and metabolism. A greater number of differentially expressed genes related to the general stress response (GSR) and biofilm formation is observed in simulated microgravity cultures under glucose-limited conditions in comparison to glucose-replete conditions. Longer term SµG culture under glucose-limited conditions led to the accumulation of unique mutations when compared to control cultures, particularly in the mraZ/fruR intergenic region and the elyC gene, suggesting changes in peptidoglycan and enterobacterial common antigen (ECA) production. These findings highlight the physiological and genomic adaptations of E. coli to microgravity, offering a foundation for future research into the long-term effects of space conditions on bacterial evolution.

Indexed as

Adaptation, PhysiologicalCell MembraneEscherichia coliStress, PhysiologicalWeightlessnessWeightlessness SimulationBiofilmsEscherichia coli ProteinsGene Expression ProfilingGene Expression Regulation, BacterialGlucoseEscherichia coli ProteinsGlucoseE. coliExperimental evolutionGene expressionREL606Simulated microgravity

Identifiers

PMID40484963
PMCPMC12147273

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.