Evidence map›Paper›PMID 40483447›Full record

ArticleBMC plant biology2025

Genetic composition and heterozygosity of gibberellin-induced 2n gametes evaluated by SSR markers in Populus.

Yifan Zhao, Yunyun Xie, Bo Kong, Dongliang Cao, Jiahua Du, Lexun Ma, Meiling Long, Liang Li, Yaru Sang, Dawei Cheng and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Understanding and harnessing unreduced gametes for crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yifan ZhaoState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Yunyun XieState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Bo KongState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Dongliang CaoState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Jiahua DuState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Lexun MaState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Meiling LongState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Liang LiState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Yaru SangState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Dawei ChengState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Huiqi LaoState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Yaqi ZhaoState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Huajian ZhangState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Xiangyang KangNational Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China.
Pingdong ZhangState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China. zhangpd@bjfu.edu.cn.

Funding

the National Key R&D Program of China during the 14th Five-year Plan Period 2021YFD2200105
6 · The paper itself

Abstract

backgroundThe artificial induction of unreduced (2n) gametes, due to first division restitution (FDR) or second division restitution (SDR), is an important method to produce triploids in higher plants. It is crucial to evaluate the genetic composition and heterozygosity transmission of induced 2n gametes with different formation mechanisms.

resultsHere, we produced 110 triploids by pollinating the female inflorescence of Populus alba × P. glandulosa with gibberellin-induced 2n pollen of Populus bolleana. The genetic composition and heterozygosity of the induced 2n pollen were inferred from SSR marker analysis of the triploid offspring, using 27 primer pairs distributed across nine Populus chromosomes. This revealed that, among the 110 hybrid triploids, 70 triploids did originate from FDR-type 2n pollen and the other 40 from SDR-type 2n pollen. The FDR-type and SDR-type 2n pollen transmitted a substantially different level of parental heterozygosity (0.7545 and 0.3167, respectively) in P. bolleana. Additionally, compared with the low coefficient of variation (CV = 12.82%) for heterozygosity transmitted by FDR-type 2n pollen, there was much higher coefficient of variation (CV = 48.82%) for that transmitted by SDR-type 2n pollen.

conclusionsOur findings provide a valuable reference point for bolstering suitable applications of 2n gametes in future research, and can help foster new strategies of triploid breeding in plants. CLINICAL TRIAL NUBMER: Not applicable.

Indexed as

Germ Cells, PlantGibberellinsMicrosatellite RepeatsPopulusChromosomes, PlantGenetic MarkersHeterozygotePollenTriploidyGenetic MarkersGibberellinsFirst division restitutionGA3-induced 2n gametesSecond division restitutionSSR markerTransmission of parental heterozygosity

Identifiers

PMID40483447
PMCPMC12144806

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.