Evidence map›Paper›PMID 40482964›Full record

ArticleJournal of advanced research2026

OsCPK9-mediated Thr105 phosphorylation activates OsCATC to enhance drought tolerance in rice.

Fu Shi, Li Li, Yaqiong Wang, Yanbin Guan, Ya'Nan Wu, Qian Zhang, Junli Chang, Mingjie Chen, Yuesheng Wang, Sanhe Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. The transcription factor ZmMYB3R targets CYCB1;2 to modulate maize drought tolerance.The Plant journal : for cell and molecular biology · 2026
    Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Fu ShiThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Li LiThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Yaqiong WangThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Yanbin GuanThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Ya'Nan WuThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Qian ZhangThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Junli ChangThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Mingjie ChenThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Yuesheng WangThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
Sanhe LiKey Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430068, China.
Xuebin ZhangState Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475004 Henan, China.
Guangyuan HeThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: hegy@hust.edu.cn.
Yin LiThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: yinli2021@hust.edu.cn.
Guangxiao YangThe Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: ygx@hust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionDrought poses a significant environmental challenge, disrupting plant growth and reducing crop productivity. As sensors and effectors in calcium signaling, calcium-dependent protein kinases (CPKs) regulate plant development and environmental adaptation. However, the specific roles and molecular networks of many OsCPKs in rice adaptation to abiotic stress remain to be elucidated.

objectivesDrought-tolerant rice holds significant potential for enhancing yield stability and global food security. The study aimed to isolate drought-responsive OsCPKs and explore their molecular mechanisms.

methodsTo elucidate the mechanisms underlying OsCPK9-mediated drought tolerance, we integrated assays of Co-immunoprecipitation, yeast two-hybrid, GST pull-down and bimolecular fluorescence complementation to validate the OsCPK9-interacting proteins. In vitro protein phosphorylation analysis was conducted to identify phosphorylated substrates and sites. Rice yield performance was evaluated through three-year field trials.

resultsOsCPK9 overexpressing plants enhanced drought tolerance in rice, accompanied by lower H

conclusionThis study reveals that myristoylation and palmitoylation of OsCPK9 contribute to its plasma membrane localization and drought tolerance. The post-translational regulatory mechanism mediated by OsCPK9 enhances environmental adaptability and provides a promising strategy to improve rice grain yield under drought stress conditions.

Indexed as

Adaptation, PhysiologicalDroughtsOryzaPlant ProteinsProtein KinasesCalcium-Calmodulin-Dependent Protein KinasesDrought ResistanceGene Expression Regulation, PlantPhosphorylationPlants, Genetically ModifiedStress, PhysiologicalThreonineCalcium-Calmodulin-Dependent Protein Kinasescalcium-dependent protein kinase, ricePlant ProteinsProtein KinasesThreonineDrought stress toleranceH(2)O(2)OsCATCOsCPK9PhosphorylationRice

Identifiers

PMID40482964
PMCPMC12957816

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.