Evidence map›Paper›PMID 40482649›Full record

ArticleCurrent biology : CB2025

Negative feedback equalizes polarity sites in a multi-budding yeast.

Alex W Crocker, Claudia A Petrucco, Kaiyun Guan, Alison C E Wirshing, Joanne L Ekena, Daniel J Lew, Timothy C Elston, Amy S Gladfelter

Erratum issuedAbstract read
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In one paragraph

Article in Current biology : CB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. A self-organizing single-cell morphology circuit optimizesbioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Alex W CrockerCurriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, 120 Mason Farm Rd., Chapel Hill, NC 27599, USA; Duke University, Department of Cell Biology, 308 Research Drive, Durham, NC 27705, USA.
Claudia A PetruccoDuke University, Department of Cell Biology, 308 Research Drive, Durham, NC 27705, USA.
Kaiyun GuanCurriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, 120 Mason Farm Rd., Chapel Hill, NC 27599, USA.
Alison C E WirshingDepartment of Biology, Massachusetts Institute of Technology, 31 Ames St., Cambridge, MA 02139, USA.
Joanne L EkenaDuke University, Department of Cell Biology, 308 Research Drive, Durham, NC 27705, USA.
Daniel J LewDepartment of Biology, Massachusetts Institute of Technology, 31 Ames St., Cambridge, MA 02139, USA.
Timothy C ElstonDepartment of Pharmacology, University of North Carolina at Chapel Hill, 120 Mason Farm Rd., Chapel Hill, NC 27599, USA; Computational Medicine Program, University of North Carolina at Chapel Hill, 116 Manning Drive, Chapel Hill, NC 27599, USA. Electronic address: telston@med.unc.edu.
Amy S GladfelterDuke University, Department of Cell Biology, 308 Research Drive, Durham, NC 27705, USA. Electronic address: amy.gladfelter@duke.edu.

Funding

Studies on cell polarity, chemotropism, and cell cycle controlR35GM122488 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI DANIEL J LEW · 2017 to 2026
$6.9M
Mathematical modeling of cellular signaling systemsR35GM127145 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Timothy C Elston · 2018 to 2026
$3.9M
Organization of Syncytial CellsR35GM156800 · NIGMS · DUKE UNIVERSITY · PI Amy S Gladfelter · 2025 to 2026
$1.1M
NIGMS NIH HHS R35 GM122488NIGMS NIH HHS R35 GM127145NIGMS NIH HHS R35 GM156800
6 · The paper itself

Abstract

Morphogenesis in fungi and animals is directed by the polarization of the small GTPases Cdc42 and Rac. In the budding yeast Saccharomyces cerevisiae, competition between polarity patches results in one polarized patch and the growth of a single bud. Here, we describe cell polarity in the yeast Aureobasidium pullulans, which establishes multiple coexisting polarity sites yielding multiple buds during a single cell-division cycle. Polarity machinery components oscillate in their abundance in these coexisting sites but do so independently of one another, pointing to a lack of global coupling between sites. Previous theoretical work has demonstrated that negative feedback in a polarity circuit could promote the coexistence of multiple polarity sites, and time-delayed negative feedback is known to cause oscillations. We show that both these features of negative feedback depend on a protein we identified as Pak1 and that Pak1 requires Rac1 but not Cdc42 for its localization. This work shows how conserved signaling networks can be modulated for distinct morphogenic programs even within the constraints of fungal budding.

Indexed as

AscomycotaCell PolarityFeedback, Physiologicalcdc42 GTP-Binding Protein, Saccharomyces cerevisiaeFungal Proteinsp21-Activated Kinasesrac1 GTP-Binding ProteinSignal Transductioncdc42 GTP-Binding Protein, Saccharomyces cerevisiaeFungal Proteinsp21-Activated Kinasesrac1 GTP-Binding ProteinCdc42extremotolerant yeastsmulti-buddingPak1polarity

Identifiers

PMID40482649
PMCPMC12237600

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.