Evidence map›Paper›PMID 40482644›Full record

ArticleCell reports. Medicine2025

Extracellular vesicle heterogeneity through the lens of multiomics.

Taylon F Silva, Elizabeth Hutchins, Wenyan Zhao, Yari Ciani, Minhyung Kim, Emily Ko, Javier Mariscal, Zhuyu Qiu, Fatima Bedier, Agnes Kittel and 20 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Condensates on the Move: Midbody Remnants as Large, Translation-Competent Extracellular Vesicles.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Review
  14. Laurdan: Clarifying Photophysics and Advancing the Characterization of Extracellular Vesicles.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026
    Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

30 authors.

Taylon F SilvaDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Elizabeth HutchinsNeurogenomics Division, Translational Genomics Research Institute (TGen), Phoenix, AZ, USA.
Wenyan ZhaoJonsson Comprehensive Cancer Center, University of California: Los Angeles, Los Angeles, CA, USA.
Yari CianiDepartment of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, Italy.
Minhyung KimDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Emily KoDepartment of Radiation Oncology, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Javier MariscalDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Zhuyu QiuJonsson Comprehensive Cancer Center, University of California: Los Angeles, Los Angeles, CA, USA; Department of Human Genetics, University of California: Los Angeles, Los Angeles, CA, USA.
Fatima BedierDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Agnes KittelHUN-REN Institute of Experimental Medicine, Budapest, Hungary.
Bo ZhouDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Yang WangDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Megan HallNeurogenomics Division, Translational Genomics Research Institute (TGen), Phoenix, AZ, USA.
Francesca GalassoNeurogenomics Division, Translational Genomics Research Institute (TGen), Phoenix, AZ, USA.
Rebecca ReimanNeurogenomics Division, Translational Genomics Research Institute (TGen), Phoenix, AZ, USA.
Michael R FreemanDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Sarah ParkerSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Jennifer Van EykSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Wei YangDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Edwin PosadasSamuel Oschin Comprehensive Cancer Institute, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Department of Medicine, David Geffen School of Medicine, University of California: Los Angeles, Los Angeles, CA, USA.
Jlenia GuarnerioDepartment of Radiation Oncology, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Department of Hematology Oncology, David Geffen Medical School, University of California: Los Angeles, Los Angeles, CA, USA.
John NolanScintillon Institute for Biomedical and Bioenergy Research, San Diego, CA, USA.
Clotilde ThéryInstitut Curie, PSL Research University, INSERMU932, Paris, France.
Andries ZijlstraDepartment of Pathology, Vanderbilt University Medical Center, Nashville, TN, USA.
Shannon StottMassachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA, USA; BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Sungyong YouDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Francesca DemichelisDepartment of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, Italy.
Paul C BoutrosJonsson Comprehensive Cancer Center, University of California: Los Angeles, Los Angeles, CA, USA; Department of Human Genetics, University of California: Los Angeles, Los Angeles, CA, USA; Department of Urology, University of California: Los Angeles, Los Angeles, CA, USA; Institute for Precision Health, University of California: Los Angeles, Los Angeles, CA, USA. Electronic address: pboutros@mednet.ucla.edu.
Kendall Van Keuren-JensenNeurogenomics Division, Translational Genomics Research Institute (TGen), Phoenix, AZ, USA. Electronic address: kjensen@tgen.org.
Dolores Di VizioDepartment of Urology, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Samuel Oschin Comprehensive Cancer Institute, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Electronic address: dolores.divizio@cshs.org.

Funding

Molecular Mechanisms of Large Oncosome-Induced Prostate Cancer Progression and MetastasisR01CA234557 · NCI · CEDARS-SINAI MEDICAL CENTER · PI DI VIZIO, DOLORES · 2019 to 2023
$2.3M
Clinical Translation of a Large Oncosome-Based Prostate Cancer Blood TestR01CA287075 · NCI · CEDARS-SINAI MEDICAL CENTER · PI DEMICHELIS, FRANCESCA, FREEMAN, MICHAEL R. · 2024 to 2025
$1.3M
NCI NIH HHS R01 CA234557NCI NIH HHS R01 CA287075
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are heterogeneous in size, biogenesis, content, and function. Aggressive cancer cells release a distinct, poorly characterized, and particularly large EV subtype, namely large oncosomes (LOs). This study employs an optimized method to improve LO yields and integrates mass spectrometry and RNA sequencing (RNA-seq) to profile their molecular cargo. A consistent set of proteins enriched in LOs is identified across glioma, prostate, and breast cancer cell lines. These proteins are also present as mRNA in LOs from the prostate cancer model and are abundant in plasma LOs from 20 patients with metastasis. Single-LO RNA-seq confirms bulk LO cargo, demonstrating the utility of single-cell technologies for large vesicle analysis. Our patient study provides proof-of-principle evidence that we can use multiomics to delve into EV heterogeneity, biogenesis, and composition. It also suggests that plasma LOs help stratify patients, supporting their potential prognostic value for developing a multi-analyte approach for liquid biopsy.

Indexed as

Extracellular VesiclesProteomicsCell Line, TumorFemaleHumansMaleMass SpectrometryMultiomicsNeoplasmsProstatic NeoplasmsSingle-Cell Analysiscancerextracellular vesicleslarge oncosomesliquid biopsymultiomicsprostate cancerproteomicsingle-vesicle RNA-seqtranscriptomic

Identifiers

PMID40482644
PMCPMC12281359

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.