Evidence map›Paper›PMID 40481419›Full record

ArticleBMC microbiology2025

Effect of photodynamic therapy with imipenem and gentamicin-derived carbon dots on the expression of quorum sensing and virulence genes in Pseudomonas aeruginosa.

Somaye Shiralizadeh, Mohammad Sina Alikhani, Abbas Farmany, Masoud Azimzadeh, Leili Shokoohizadeh, Maryam Pourhajibagher, Abbas Bahador, Mohammad Yousef Alikhani

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In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Somaye ShiralizadehStudents Research Committee, Hamadan University of Medical Sciences, Hamadan, IR, Iran.
Mohammad Sina AlikhaniStudents Research Committee, Hamadan University of Medical Sciences, Hamadan, IR, Iran.
Abbas FarmanyDental Implant Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
Masoud AzimzadehStudents Research Committee, Hamadan University of Medical Sciences, Hamadan, IR, Iran.
Leili ShokoohizadehDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR, Iran.
Maryam PourhajibagherDental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Abbas BahadorDepartment of Microbiology, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Yousef AlikhaniInfectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, IR, Iran. alikhani43@yahoo.com.ORCID 0000-0003-4577-4029

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe capability of Pseudomonas aeruginosa to cause various severe infections is linked to the production of various virulence factors, regulated by quorum sensing. The objective of this study was to assess the effect of sub-lethal doses of antimicrobial photodynamic inactivation (sAPDI), employing carbon dots (CDs) derived from gentamicin and imipenem, on the expression of genes in two key quorum sensing systems (the rhl and las systems) and on the genes responsible for producing elastase (lasB), protease (lasA), rhamnolipid (rhlA), and pyocyanin (phzM) in P. aeruginosa.

methodsCDsGEN-NH2, and CDsIMP-NH2 were synthesized from gentamicin and imipenem precursors through the hydrothermal method. The Conjugation of imipenem to CDsGEN-NH2 (CDsGEN-IMP) and gentamicin to CDsIMP-NH2 (CDsIMP-GEN) was done. The study evaluated the effect of sAPDI at sub-minimum biofilm inhibitory concentrations (sub-MBIC) of CDs, combined with a sub-lethal dose of UVA light, on the expression of QS and virulence encoding genes using real-time quantitative polymerase chain reaction (RT-qPCR).

resultsFollowing treatment with sAPDI, there was a notable decline in the expression levels of QS and virulence genes compared to the control (P < 0.05). Conversely, treatment with sub-MBIC concentrations of CDs and a sub-lethal dose of UVA light irradiation alone did not significantly alter the expression of these genes relative to the control group (P > 0.05).

conclusionThe findings indicate that sAPDI, employing CDs sourced from imipenem and gentamicin combined with UVA light exposure, leads to a decrease in the expression of genes related to quorum sensing and virulence in P. aeruginosa.

Indexed as

Anti-Bacterial AgentsGentamicinsImipenemPhotochemotherapyPseudomonas aeruginosaQuorum SensingVirulence FactorsBacterial ProteinsBiofilmsCarbonCarbon Quantum DotsGene Expression Regulation, BacterialMicrobial Sensitivity TestsVirulenceAnti-Bacterial AgentsBacterial ProteinsCarbonGentamicinsImipenemVirulence FactorsAntimicrobial photodynamic inactivationCarbon dotsGene expressionPseudomonas aeruginosaQuorum sensingVirulence factors

Identifiers

PMID40481419
PMCPMC12144813

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.