Evidence map›Paper›PMID 40481046›Full record

ArticleScientific reports2025

Isolation and characterization of human cKIT positive amniotic fluid stem cells obtained from pregnancies with spina bifida.

Katarzyna Michalak-Micka, Nadine Huerlimann, Nicole Ochsenbein-Kölble, Martin Ehrbar, Agnes Silvia Klar, Thomas Biedermann, Ueli Moehrlen

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Katarzyna Michalak-MickaTissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, Zurich, 8032, Switzerland.
Nadine HuerlimannTissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, Zurich, 8032, Switzerland.
Nicole Ochsenbein-KölbleThe Zurich Center for Fetal Diagnosis and Therapy, University of Zurich, Zurich, Switzerland.
Martin EhrbarUniversity of Zurich, Zurich, Switzerland.
Agnes Silvia KlarTissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, Zurich, 8032, Switzerland.
Thomas BiedermannTissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, Zurich, 8032, Switzerland.
Ueli MoehrlenTissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, Zurich, 8032, Switzerland. Ueli.Moehrlen@kispi.uzh.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Myelomeningocele (MMC) and myeloschisis (MS) are considered as the most severe forms of spina bifida aperta characterized by incomplete closure of the neural tube during the first trimester of pregnancy. Open maternal-fetal surgery has been identified as a promising alternative for the repair of MMC/MS. The main goal of this study was the isolation and characterization of stem cells derived from MMC/MS amniotic fluid samples. Human amniotic fluid samples were obtained from pregnant women who underwent surgery for fetal spina bifida repair. We applied fluorescence activated-cell sorting (FACS) to immunoselect stem cells from heterogeneous populations of amniocytes based on cKIT (CD117) expression. The cKIT

Indexed as

Amniotic FluidProto-Oncogene Proteins c-kitSpinal DysraphismStem CellsAdultCell SeparationFemaleFlow CytometryHumansPregnancyKIT protein, humanProto-Oncogene Proteins c-kitAmniotic fluidAmniotic fluid stem cellsMyelomeningoceleMyeloschisisSpina bifida aperta

Identifiers

PMID40481046
PMCPMC12144188

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.