Evidence map›Paper›PMID 40480306›Full record

ArticleDevelopmental biology2025

VEGF signaling promotes blastema growth and proliferation of vascular and non-vascular cells during axolotl limb regeneration.

Aaron M Savage, Alexandra C Wagner, Ryan T Kim, Paul Gilbert, Hani D Singer, Erica Chen, Elane M Kim, Noah Lopez, Kelly E Dooling, Julia C Paoli and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Aaron M SavageDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Alexandra C WagnerDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Ryan T KimDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Paul GilbertDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Hani D SingerDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Erica ChenDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Elane M KimDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Noah LopezDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Kelly E DoolingDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Julia C PaoliDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
S Y Celeste WuDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Sebastian BöhmDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Tim FroitzheimDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Rachna ChilambiDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Steven J BlairDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Connor J PowellDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Adnan AbouelelaDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Anna G LuongDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Kara N ThorntonDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Noora HarakeDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Alparslan KarabacakDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Benjamin TajerDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Duygu Payzin-DogruDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Jessica L WhitedDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA; The Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA. Electronic address: jessica_whited@harvard.edu.

Funding

IDENTIFYING ROADBLOCKS TO LIMB REGENERATIONR01HD095494 · NICHD · HARVARD UNIVERSITY · PI WHITED, JESSICA L. · 2019 to 2023
$1.9M
NICHD NIH HHS R01 HD095494
6 · The paper itself

Abstract

Salamanders are capable of regenerating whole limbs throughout life, a feat that is unmatched by other tetrapods. Limb regeneration is dependent upon the formation of a blastema containing progenitor cells which give rise to most tissues of the regenerated limb. Many signaling pathways, including FGF, BMP and Wnt, are required for regeneration, but the role of VEGF signaling during salamander limb regeneration is not well understood, particularly outside of angiogenesis. Here we show that VEGF signaling is essential for limb regeneration and that blastema cells and limb fibroblasts display impaired proliferation in the absence of VEGF signaling. Loss of VEGF signaling reduces expression of EMT-associated genes, suggesting VEGF signaling promotes expression of EMT-associated transcription factors, including Snai2, during axolotl limb regeneration. These findings highlight potential roles for VEGF signaling during regeneration which may extend beyond its expected pro-angiogenic function.

Indexed as

Ambystoma mexicanumExtremitiesRegenerationSignal TransductionVascular Endothelial Growth Factor AAnimalsCell ProliferationFibroblastsNeovascularization, PhysiologicVascular Endothelial Growth Factor A

Identifiers

PMID40480306
PMCPMC12371191

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.